A multiplex PCR assay was developed for detection of the six types of glycopeptide resistance characterized in enterococci and for identification of Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis at the species level. Primers targeting the genes vanA, vanB, vanC, vanD, vanE, vanG, and ddl of E. faecium and E. faecalis and nuc of S. aureus and ...

A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11 pulsed-field gel electrophoresis types; 17 contained the prototype vanB2 and 8 contained a novel vanB2 with a 177-bp deletion in vanY(B). Both vanB2 genes were transmissible in vitro at a mean frequenc...

aim & background : enterococci infections are public health growing concern due to the glycopeptide antibiotics resistance especially vancomycin. genes, vana, b, and h, are contributed to the influence of vancomycin-resistant enterococci (vre). this investigation aims at exploring the vre frequency and the rate of each genes in isolated enterococci from gastroenteritis patients in central regio...

Enterococci are part of the normal human flora. Enterococcus faecalis und Enterococcus faecium are the most common species isolated from human infections. Vancomycinresistant enterococci (VRE) have emerged as a major cause of nosocomial infection as early as 1986 [1]. 10 years later, >15% of nosocomial enterococcal infections in United States hospitals were due to VRE, and even higher in intens...

We examined 105 clinical isolates of glycopeptide-resistant enterococci collected from 31 U.S. hospitals in 14 states during May 1988 to July 1992. The isolates included 82 Enterococcus faecium, 8 E. faecalis, 6 Enterococcus spp., 5 E. gallinarum, 3 E. casseliflavus, and 1 E. raffinosus. The isolates were categorized into the following four phenotypes of glycopeptide resistance on the basis of ...