Cholera is an intestinal infection caused by Vibrio cholerae. The hallmark of the disease is profuse secretory diarrhea. Cholera can be endemic, epidemic, or pandemic. A total of 15 Vibrio cholerae isolates were obtained from diarrheal samples by standard bacteriological and serological methods. After DNA extraction, a polymerase chain reaction (PCR) assay was performed for detection of the pre...

Objective(s): Listeria monocytogens, Bacillus cereus and Campylobacter jejuni are three toxin producing bacteria over the world, especially in Iran, and it is essential to find a certain, rapid procedure to identify these microorganisms. In this research, these bacteria were simultaneously detected by multiplex PCR technique in foods. Materials and Methods: The primary approval of bacterial str...

Abstract Background Cost effectiveness was a criterion used to revise Ethiopia’s essential health service package (EHSP) in 2019. However, there are few cost-effectiveness studies from Ethiopia or directly transferable evidence other low-income countries inform comprehensive revision of the Ethiopian EHSP. Therefore, this paper reports average ratios (ACERs) 159 interventions Methods In study, ...

The hly-encoded listeriolysin O (LLO) is a major virulence factor secreted by the intracellular pathogen Listeria monocytogenes, which plays a crucial role in the escape of bacteria from the phagosomal compartment. Here, we identify a putative PEST sequence close to the N-terminus of LLO and focus on the role of this motif in the biological activities of LLO. Two LLO variants were constructed: ...

Escherichia coli hemolysin, a transmembrane pore-forming exotoxin, is considered an important virulence factor for E. coli-related extraintestinal infections and sepsis. The possible significance of hemolysin liberation for induction of inflammatory lipid mediators was investigated in isolated rabbit lungs infused with viable bacteria (concentration range, 10(4)-10(7)/ml). Hemolysin-secreting E...