Human synovial tissue cells in monolayer can be shown to take up and digest a soluble protein, horseradish peroxidase (HRP). Uptake of HRP was linear with increasing concentrations of substrate and cell protein and with time for up to 4 h. Low temperature (4 degrees C), and sodium fluoride, an inhibitor of glycolysis were the most effective metabolic inhibitors of endocytosis. In addition, colc...

BACKGROUND Zoonotic cutaneous leishmaniasis (ZCL) is an increasing public health problem in some endemic regions. Horseradish peroxidase (HRP) conjugated rabbit anti-Rhombomys opimus (R. opimus) Ig is needed for immunoblotting and ELISA tests used to explore the immune response of the rodents against the sand fly saliva. In this study, the production of HRP conjugated rabbit anti-R. opimus Ig w...

Cells of the 16-cell mouse embryo endocytose horseradish peroxidase (HRP) which becomes localized in most cases to a juxtanuclear position. Cells that have ingested HRP in intact embryos, and cells dissociated from embryos prior to culture in HRP, showed similar patterns of cytoplasmic distribution of the ingested enzyme. Cells in the embryo in situ were incubated in HRP, and then labelled with...

Ultrawideband (UWB) wireless communication technology, which appeared 20 years ago, is becoming popular in various fields because the ability to measure precise distance between UWB devices beyond being re-examined. The currently commercialized technology high-rate pulse repetition frequency (HRP) uses high-speed pulses of several tens megahertz, and direction measurements are made by using cha...

background: in this study, we evaluated the protective effects of water hyacinth root powder (hrp) on arsenic-mediated toxic effects in mice. methods: swiss albino mice, used in this study, were divided into four different groups (for each group n=5). the control group was supplied with normal feed and water, arsenic group (as-group) was supplied with normal feed plus arsenic (sodium arsenite)-...

In parotid acinar cells, horseradish peroxidase (HRP) administered via the main excretory duct is endocytosed from the apical cell surface in smooth C- or ring-shaped vesicles (Oliver, C. and A. R. Hand. 1979. J. Cell Biol. 76:207). These vesicles ultimately fuse with lysosomes adjacent to the Golgi apparatus. The present investigation extends these findings and examines the uptake and fate of ...

The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends on the Hrp (hypersensitive response and pathogenicity) type III protein secretion system and the effector proteins it translocates into plant cells. Through iterative application of experimental and computational techniques, the DC3000 effector inventory has been substantially enlarged. Five homologs of kno...

Flow-based coiled systems, through which a heat transfer fluid (such as glycerol) is pumped, are widely used for thermal stabilization of bioreactors and biosensor cuvettes cells. Previously, using horseradish peroxidase (HRP) model protein, we have demonstrated that the incubation protein solution in flow-based system over pipe with flowing glycerol leads to change adsorption properties macrom...

مگس مینوز سبزی و صیفی Liriomyza sativae Blanchard ، از مهم­ترین آفات ‌های گلخانه ای فضای باز، محسوب می شود. با توجه به توصیه کاربرد حشره­ کش تیوسیکلام جهت مدیریت آفت مزبور، در پژوهش حاضر، اثرات کشندگی زیرکشندگی(5/131:LC30، 1/87:LC20، 15/49:LC10 میلی گرم ماده موثره بر لیتر) حشره ­کش (اویسکت®SP50%) روی فراسنجه ­های زیستی این شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. روش استفاده انجام آزمایش­ها غوط...