Processing of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5 could be mediated by the catalytically active NS3 protease domain and NS2B in trans at the dibasic sites NS3-NS4A and NS4B-NS5. Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution ...

Background: HIV/AIDS is still a major public health issue around the world, especially in Sub-Saharan Africa (SSA), where this has resulted unquantifiable human suffering, social and cultural disruption, enormous economic losses. Methods: This study aims to assess women’s knowledge attitude toward its prevention Ghana.<ns3:...

Many viral proteins undergo proteolytic processing events that are required for virus infection and virion assembly. In this issue of Biochemical Journal, Strongin and co-workers report that the NS3 protease from West Nile virus unexpectedly cleaves certain substrates at pairs of basic residues, a specificity that resembles that of the furin-like PCs (proprotein convertases). This led to the de...

Background: Reactive oxygen species and other free radicals, together with glucose its metabolites are believed to play important roles in the aging process. The carbohydrate components of glycosylated proteins mediating cell-cell interactions a role has been suggested for them Erythrocytes critical cells human body, heavily relatively easily available so good candid...

Background: Epilepsy affects 70 million people worldwide, 80% of whom are in low-and-middle income countries (LMICs). Infections the central nervous system (CNS) contribute considerably to burden epilepsy LMICs, but nature and presentation following these infections is not fully understood. We examined if foutcomes associated with exposure parasitic infections.</ns3:...

Objective: The objective of this scoping review is to map, from wound assessment tools and other literature, the current methods used assess odour in order answer following question: Which assessment, validated or otherwise, are currently odour? Introduction: Wound includes not only details condition bed but also evaluation symptom...

Objective: To investigate the effect of sequence variability between different genotypes of hepatitis C virus (HCV) on the antigenic properties of the NS3 proteins, and to evaluate the significance of the proteins in the serological diagnosis. Methods: The recombinant expression plasmids of pET/NS31 and pET/NS3-6 were constructed with the NS3 region fragments from HCV genotypes 1 and 6, respect...

BACKGROUND The hepatitis C virus (HCV) genome encodes a long polyprotein, which is processed by host cell and viral proteases to the individual structural and non-structural (NS) proteins. HCV NS3/4A serine proteinase (NS3/4A) is a non-covalent heterodimer of the N-terminal, ∼180-residue portion of the 631-residue NS3 protein with the NS4A co-factor. NS3/4A cleaves the polyprotein sequence at f...

The viruses of the family Flaviviridae possess a positive-strand RNA genome and express a single polyprotein which is processed into functional proteins. Initially, the nonstructural (NS) proteins, which are not part of the virions, form complexes capable of genome replication. Later on, the NS proteins also play a critical role in virion formation. The molecular basis to understand how the sam...

Background Learning to thinking critically about health information and choices can protect people from unnecessary suffering, harm, resource waste. Earlier work revealed that children learn these skills, but printing costs curricula compatibility remain important barriers school implementation. We aimed develop a set of digital learning resources for ...