BACKGROUND Quantitative reverse transcription-PCR (RT-PCR) used to detect small changes in specific mRNA concentrations is often associated with poor reproducibility. Thus, there is a need for stringent quality control in each step of the protocol. METHODS Real-time PCR-based calibration curves for a target gene, tissue factor (TF), and a reference gene, beta-actin, generated from PCR amplico...

With traditional 2-color Förster Resonance Energy Transfer (FRET) microscopy, valuable quantitative analyses can be conducted. Correlations of donor (D), acceptor (A) and their ratios (D:A) with energy transfer efficiency (E%) or distance (r) allows measurement of changes between control and experimental samples; also, clustered vs. random assembly of cellular components can be differentiated. ...

BACKGROUND Few studies have investigated predictors of discordance between liver biopsy (LB) and liver stiffness measurement (LSM) using FibroScan®. We assessed predictors of discordance between LB and LSM in chronic hepatitis B (CHB) and investigated the effects of necroinflammatory activity. METHODS In total, 150 patients (107 men, 43 women) were prospectively enrolled. Only LSM with ≥ 10 v...

A sampling design method that seeks chlorine sampling locations using a multiobjective optimization framework is proposed here. The design problem is formulated as a threeand two-objective optimization where the parameter estimation accuracy (F1) is maximized, the the number of sensors is minimized (F2) and the weighted nodal sensitivity is maximized (F3). Trade-off curves are generated using t...

lipases are versatile biocatalysts with a wide range of application in food, dairy, leather, paper, pharmaceutical and detergent industries.  in this study, mesophilic lipase gene from an indigenous bacillus pumilus f3 that already its gene had been sequenced and  identified, was cloned and expressed in methylotrophic yeast pichia pastoris and codon translation of lipase gene was evaluated in p...

The amount and distribution of intranuclear suggested the presence of intranuclear hehemoglobin in erythroblasts of normal submoglobin precipitation in some cells. jects and patients with homozygous Similar characteristic absorption peaks of thalassemia were studied by microspecdenatured ferric hemoglobin were recorded trophotometric methods. The mean intranuover large inclusions of the cytopla...

We described a method for long-term passive measurements of unattached fraction fp of the potential alpha energy concentration of radon progeny from a set of measured (f1, f2, f3) values, where fi=Ci/C0 (i=1, 2, 3), and C0, C1, C2 and C3 were the concentrations of 222Rn, and the airborne concentrations of 218Po, 214Pb and 214Bi, respectively. Jacobi room model parameters were randomly sampled f...