Intracellular vesicular trafficking and membrane fusion are important processes for nervous system development and for the function of neural circuits. Synaptosomal-associated protein 25 kDa (SNAP-25) is a component of neural soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) core complexes that mediate the exocytotic release of neurotransmitters at chemical synapses....

The effect of nitric oxide (NO) on Na+/H+ exchange (NHE) activity was investigated utilizing Caco-2 cells as an experimental model. Incubation of Caco-2 cells with 10(-3) M S-nitroso-N-acetylpenicillamine (SNAP), a conventional donor of NO, for 20 min resulted in a approximately 45% dose-dependent decrease in NHE activity, as determined by assay of ethylisopropylamiloride-sensitive 22Na uptake....

La investigación hace un recorrido desde la creación de Bellavista en década los noventa, como una iniciativa que combina conservación y el ecoturismo, hasta su declaratoria primera área protegida privada del Sistema Nacional Áreas Protegidas (SNAP) 2019, siguiendo procedimiento técnico-normativo establecido por Autoridad Ambiental. El objetivo fue documentar proceso llevó a propietarios conser...

A snap-stabilizing protocol guarantees that the system always behaves according to its specification provided some processor initiated the protocol. We present how to snap-stabilize some important protocols, like Leader Election, Reset, Snapshot, and Termination Detection. We use a Snap-stabilizing Propagation of Information with Feedback protocol for arbitrary networks as the key module in the...

We have examined the effect of alpha-SNAP on exocytosis in adrenal chromaffin cells by direct assay of exocytosis using patch-clamp capacitance analysis. Cells were recorded using the whole cell patch-clamp configuration and the cells dialysed with control pipette solution or with a pipette solution containing alpha-SNAP or the deletion mutant alpha-SNAP(41-295). The deletion mutant was found t...

The core human U6 promoter consists of a proximal sequence element (PSE) located upstream of a TATA box. The PSE is recognized by the snRNA-activating protein complex (SNAP(c)), which consists of five types of subunits, SNAP190, SNAP50, SNAP45, SNAP43, and SNAP19. The TATA box is recognized by TATA box binding protein (TBP). In addition, basal U6 transcription requires the SANT domain protein B...