Non-typhoidal Salmonella includes thousands of serovars that are leading causes of foodborne diarrheal illness worldwide. In this study, we constructed three bivalent vaccines for preventing both Salmonella Typhimurium and Salmonella Newport infections by using the aspartate semialdehyde dehydrogenase (Asd)-based balanced-lethal vector-host system. The constructed Asd+ plasmid pCZ11 carrying a ...

Corresponding author: Mr James Fielding, Communicable Disease Control Branch, Department of Human Services, PO Box 6, Rundle Mall, Adelaide SA 5000. Telephone: +61 8 8226 7177. Facsimile: +61 8 8226 7187. Email: james.fi [email protected] On 3 September 2003 the Australian Salmon ella Reference Centre in Adelaide notifi ed the Communicable Disease Control Branch, Department of Human Services...

Four strains of Salmonella typhimurium avirulent for mice were found to be capable of acting as gene acceptors in transduction experiments. An attempt was made to transduce virulence to three strains, using a typical mouse-virulent strain of S. typhimurium as donor. Two of the strains which are adenine-dependent were successfully made virulent by transduction and simultaneously became adeninein...

About 600 compounds were screened as possible carbon or nitrogen sources for Salmonella typhimurium LT-2. About 100 utilizable compounds were found.

Murine monoclonal antibodies to target Salmonella typhimurium flagellin and lipopolysaccharide (LPS) were prepared and characterized. For the immunological detection of S. typhimurium, different pairs of monoclonal antibodies (MAbs) were tested in a sandwich enzyme linked immunosorbent assay (ELISA) format. After comparison, a sandwich ELISA and immunochromatographic strip based on LPS MAbs was...

Peritoneal cells obtained from mice immunized 15 or 30 days previously with (i) 0.1 LD(50) of attenuated Salmonella typhimurium (RIA), (ii) 20 mug (dry weight) of heat-killed Salmonella (SR-11), (iii) 20 mug (dry weight) of immunogenic ribosomal subfractions, or (iv) 20 mug of ribonucleic acid (RNA) subfractions were passively transferred to normal unimmunized mice. The ability of the recipient...

While serotyping and phage typing have been used widely to characterize Salmonella isolates, sensitive subtyping methods that allow for evolutionary analyses are essential for examining Salmonella transmission, ecology, and evolution. A set of 25 Salmonella enterica isolates, representing five clinically relevant serotypes (serotypes Agona, Heidelberg, Schwarzengrund, Typhimurium, and Typhimuri...