Three indirect methods for the quality control of olanzapine (OLP) in commercial formulations based on titrimetric and spectrophotometric techniques are described using N-bromosuccinimide (NBS) and two dyes, namely amaranth and janus green B as reagents. In titrimetry, a measured excess of NBS is added to an acidified solution of OLP and the unreacted NBS is determined iodometrically. Spectroph...

Promyelocytic leukemia nuclear bodies (PML NBs) have been proposed to be involved in tumor suppression, viral defense, DNA repair, and/or transcriptional regulation. To study the dynamics of PML NBs during mitosis, we developed several U2OS cell lines stably coexpressing PML-enhanced cyan fluorescent protein with other individual marker proteins. Using three-dimensional time-lapse live cell ima...

The most represented group of resistance genes are those of the nucleotide binding site-leucine-rich repeat (NBS-LRR) class. These genes are very numerous in the plant genome, and they often occur in clusters at specific loci following gene duplication and amplification events. To date, hundreds of resistance genes and relatively few quantitative trait loci for plant resistance to pathogens have ...

Phytochromes are red- and far-red-sensing photoreceptors that detect the quantity, quality, and duration of light throughout the entire life cycle of plants. Phytochromes accumulate in the cytoplasm in the dark. As one of the earliest responses after light illumination, phytochromes localize to the nucleus where they become associated with discrete nuclear bodies (NBs). Here, we describe the st...

Promyelocytic leukemia nuclear bodies (PML-NBs) are discrete interchromosomal macromolecular structures. The integrity of this dynamic nuclear subcompartment critically depends on the presence of the name-giving PML protein. Among the permanent or transient residents of PML-NBs are various regulatory proteins, including Sp100, CBP, pRb, HIPK2, RAD51 and p53. PML-NBs are frequently targeted by v...

DISEASE resistance gene cloning is one of the main targets in modern sugarcane research. In this study, several primers were designed according to the conserved motifs in the NBS regions of known resistance genes to amplify sequences from cDNA of sugarcane variety NCo376 by polymerase chain reaction (PCR). In total, six NBS-LRR type resistance gene analogs (RGAs) were cloned, for which the dedu...

The Arabidopsis genome contains approximately 200 genes that encode proteins with similarity to the nucleotide binding site and other domains characteristic of plant resistance proteins. Through a reiterative process of sequence analysis and reannotation, we identified 149 NBS-LRR-encoding genes in the Arabidopsis (ecotype Columbia) genomic sequence. Fifty-six of these genes were corrected from...

BACKGROUND Many inborn errors of metabolism (IEMs) may present as sudden infant death (SID). Nowadays, increasing numbers of patients with IEMs are identified pre-symptomatically by population neonatal bloodspot screening (NBS) programmes. However, some patients escape early detection because their symptoms and signs start before NBS test results become available, they even die even before the ...

The UK National Blood Service (NBS) is a public funded body that is responsible for distributing blood and associated products. A discrete-event simulation of the NBS supply chain in the Southampton area has been built using the commercial off-the-shelf simulation package (CSP) Simul8. This models the relationship in the health care supply chain between the NBS Processing, Testing and Issuing (...