Cleavage of C5 by C5 convertase is the last enzymatic step in the complement activation cascade leading to the formation of the cytolytic proteolytically activated form of C5 (C5b)-9 complex. In the present study, we examined the effect of the density of C3b (the proteolytically activated form of C3) on the function of the noncatalytic subunit of natural surface-bound forms of the enzyme. A com...

Transgenic mice expressing a major histocompatibility complex class II-restricted T cell receptor with specificity for a natural self-antigen, the fifth component of complement, were generated to analyze the mechanism of tolerance induction to a blood-borne self-protein. In the absence of C5 protein thymocytes from T cell receptor transgenic mice develop into mature CD4 single positive cells wh...

OBJECTIVE The purpose of this study was to elucidate the pathological characteristics of C3-C4 cervical spondylotic myelopathy (CSM) from the radiological study. MATERIALS AND METHODS A total of 31 patients with single level anterior cervical discectomy and fusion (ACDF) at C3-C4 and 46 patients with single level ACDF at C5-C6 were included in this study. We selected C5-C6 level as a represen...

STUDY DESIGN Retrospective review. OBJECTIVE To evaluate key risk factors for the development of C5 palsy after cervical corpectomy, including resection of the posterior longitudinal ligament (PLL). SUMMARY OF BACKGROUND DATA Postoperative C5 palsy is a well-known complication after cervical spine surgery. It is unknown whether resection of the PLL affects the incidence of C5 palsy. METHO...

We compute the free energy density F for gauge theories, with fermions, at high temperature and zero chemical potential. In the expansion F = T [c0 + c2g 2 + c3g 3 + (c4 ln g + c4)g + (c5 ln g+ c5)g +O(g)], we determine c5 and c5 analytically by calculating twoand three-loop diagrams. The g term constitutes the first correction to the g term and is for the non-Abelian case the last power of g t...

We compute the free energy density F for gauge theories, with fermions, at high temperature and zero chemical potential. In the expansion F = T [c0 + c2g + c3g + (c4 ln g + c4)g + (c5 ln g+ c5)g 5 +O(g)], we determine c5 and c5 analytically by calculating twoand three-loop diagrams. The g term constitutes the first correction to the g term and at the same time is the last power of g that can be...

The molecular and pharmacological characteristics of muscarinic receptor subtypes in the rat parotid acinar cell line, PAR-C5, were determined and compared with native rat parotid glands to evaluate the PAR-C5 cell line as a model to study receptor-mediated secretion. Reverse transcription-polymerase chain reaction (RT-PCR) identified mRNAs for M(3), M(4), and M(5) receptor subtypes in both PAR...

OBJECTIVE Our aim was to identify the impact of different surgical strategies on the incidence of C5 palsy. BACKGROUND Degenerative cervical spinal stenosis is a steadily increasing morbidity in the ageing population. Postoperative C5 nerve root palsy is a common complication with severe impact on the patients´ quality of life. METHODS We identified 1708 consecutive patients who underwent c...

RATIONALE Reports from our laboratory, as well as those from others, have documented the importance of complement activation, the C3a anaphylatoxin, and its receptor, C3aR, in promoting Th2 effector functions in a mouse model of bronchopulmonary allergy. Although deficiency in the fifth complement component (C5) has been linked to enhanced airway hyperresponsiveness in mice, the contribution of...

An mAb was raised to the C5 phagosomal antigen in Paramecium multimicronucleatum. To determine its function, the cDNA and genomic DNA encoding C5 were cloned. This antigen consisted of 315 amino acid residues with a predicted molecular weight of 36,594, a value similar to that determined by SDS-PAGE. Sequence comparisons uncovered a low but significant homology with a Schizosaccharomyces pombe ...