A facilitated transport process that removes the endogenous cannabinoid anandamide from extracellular spaces has been identified. Once transported into the cytoplasm, fatty acid amide hydrolase (FAAH) is responsible for metabolizing the accumulated anandamide. We propose that FAAH contributes to anandamide uptake by creating and maintaining an inward concentration gradient for anandamide. To ex...

the aim of this study was to determine the chemical constituents, antimicrobial, cytotoxicity, mutagenic and anti-mutagenic activities of the essential oil of artemisia ciniformis krasch. & popov ex poljakov, against important bacterial pathogens and human cells which were unknown before. in vitro cytotoxicity was measured using a modified mtt assay on normal human lymphocytes and tumor hela ce...

it has been reported that several members of convolvulaceae family such as convolvulus arvensis possess cytotoxic activity on some tumor cell lines. proteoglycan mixture, the water extract of c. arvensis with high molecular weight, has potent anti-angiogenesis and stimulating effects. in this study, arial parts of c. arvensis were collected and identified, and the cytotoxic effects of chlorofor...

Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase (HDAC) inhibitor which has anticancer effects. We evaluated the growth inhibitory effects of SAHA on HeLa cervical cancer cells in relation to reactive oxygen species (ROS) levels. SAHA inhibited the growth of HeLa cells with an IC(50) of approximately 10 µM at 24 h, and induced apoptosis which was accompanied by the cleavage of PA...

) Cancer stem cells (CSCs) are believed responsible for the tumor initiation and the resistance to current cancer therapies such as chemoand radiotherapy, which also lead to tumor recurrences and metastasis. By using a 3-D sphere culture method that favors the growth of self-renewal cells, we have successfully isolated sphere forming cells (SFCs) from cervical cancer HeLa and SiHa cells. It is ...

The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

When chick erythrocyte nuclei are introduced into the cytoplasm of HeLa cells they resume the synthesis of DNA. The reactivated nuclei synthesize DNA in a highly co-ordinated manner and essentially in synchrony with the HeLa nuclei. Asynchronous DNA synthesis is rare. The erythrocyte nuclei in the HeLa cytoplasm respond to the signals which regulate DNA synthesis in the same way as the HeLa nuc...

Reactive oxygen species (ROS) act as a mediator of ionizing radiation-induced cellular damage. Previous studies have indicated that MnSOD (SOD2) plays a critical role in protection against ionizing radiation in mammalian cells. In this study, we constructed two types of stable HeLa cell lines overexpressing SOD2, HeLa S3/SOD2 and T-REx HeLa/SOD2, to elucidate the mechanisms underlying the prote...