Virosecurinine is a major alkaloid of the plant Securinega suffruticosa and has been found to be a potent agent in inducing the differentiation of cancer cells. The present study aimed to investigate the antitumor effects of virosecurinine by inducing the apoptosis of leukemic K562 cells and to examine the underlying mechanisms. K562 cells were treated with different concentrations of virosecur...

مگس مینوز سبزی و صیفی Liriomyza sativae Blanchard ، از مهم­ترین آفات ‌های گلخانه ای فضای باز، محسوب می شود. با توجه به توصیه کاربرد حشره­ کش تیوسیکلام جهت مدیریت آفت مزبور، در پژوهش حاضر، اثرات کشندگی زیرکشندگی(5/131:LC30، 1/87:LC20، 15/49:LC10 میلی گرم ماده موثره بر لیتر) حشره ­کش (اویسکت®SP50%) روی فراسنجه ­های زیستی این شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. روش استفاده انجام آزمایش­ها غوط...

The purpose of this study was to analyze the effects of bufalin on the gene expression of K562 cells and on the expression of BMI‑1 pathway constituents in K562 cell apoptosis. K562 cells were treated with bufalin, and the inhibition rate and apoptosis were detected by an MTT assay, flow cytometry and a microarray assay. BMI‑1, p16INK4a and p14ARF were examined by quantitative polymerase chain ...

In this study, we have explored the possibility of the combination of the high reactivity of nano Fe3O4 or Au nanoparticles and daunomycin, one of the most important antitumor drugs in the treatment of acute leukemia clinically, to inhibit MDR of K562/A02 cells. Initially, to determine whether the magnetic nanoparticle Fe3O4 and Au can facilitate the anticancer drug to reverse the resistance of...

BACKGROUND The aim of this study was to investigate the anticancer effect and related mechanisms of gambogic acid (GA), a traditional Chinese medicine, on human leukemia cell line K562, together with the effect on bone marrow mononuclear cells (MNCs). MATERIAL AND METHODS K562 cells and MNCs were treated with various concentrations and treatment times of GA. Inhibitory rate was detected by us...

objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...

Human polymorphonuclear leukocytes (PMN) and granule-free cytoplasts were compared for their cytotoxic capacities against red blood cells (RBC) and K562 tumor cells. Phorbol myristate acetate (PMA) stimulated PMN to efficient lysis of RBC targets, while cytotoxicity against the tumor cell line K562 was moderate. Activated cytoplasts also lysed RBC targets but were not able to kill K562 tumor ce...

Chronic myeloid leukaemia (CML) is a form of leukaemia derived from the myeloid cell lineage. Imatinib mesylate, the breakpoint cluster region-abelson murine leukeamia kinase inhibitor, is a specific reagent used in the clinical treatment of CML. The DNA topoisomerase II inhibitor, etoposide, is also employed as a therapeutic, though it is used to a lesser extent. The present study aims to eval...