The advent of next generation sequencing technologies (NGS) has expanded the area of genomic research, offering high coverage and increased sensitivity over older microarray platforms. Although the current cost of next generation sequencing is still exceeding that of microarray approaches, the rapid advances in NGS will likely make it the platform of choice for future research in differential g...

The distribution of Ilarvirus species populations amongst 61 Australian Prunus trees was determined by next generation sequencing (NGS) of amplicons generated using a genus-based generic RT-PCR targeting a conserved region of the Ilarvirus RNA2 component that encodes the RNA dependent RNA polymerase (RdRp) gene. Presence of Ilarvirus sequences in each positive sample was further validated by Sa...

Background: Osteogenesis imperfecta (OI) is a clinically and genetically heterogeneous disorder characterized by bone loss and bone fragility. The aim of this study was to investigate the variants of three genes involved in the pathogenesis of OI. Methods: Molecular genetic analyses were performed for COL1A1, COL1A2, and CRTAP genes in an Iranian family with OI. The DNA samples were analyzed by...

The clinical application of next-generation sequencing (NGS) as a diagnostic tool has become increasingly evident. The coupling of NGS technologies with new genomic sequence enrichment methods has made the sequencing of panels of target genes technically feasible, at the same time as making such an approach cost-effective for diagnostic applications. In this article, we discuss recent studies t...

Overview of NGS technology In the decade since the completion of the human genome project, DNA sequencing technology has changed considerably. The commercial availability of the first massively parallel pyrosequencing platform in 2005 led to a new era of high-throughput genomic analysis, now referred to as next-generation sequencing (NGS). NGS technologies use a fundamentally different approach...

The full arrival and broader availability of next-generation sequencing (NGS) is transforming the practice of medicine, including neurology. Compared with the traditional one-gene-at-a-time Sanger sequencing, NGS, or massively parallel sequencing, is a radically different approach to genetic sequencing. NGS allows for a large number of genes to be captured and sequenced in parallel, creating an...

Radiant Genomics and Labcyte have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo® 525 Liquid Handler. In addition to cost savings, we demonstrate an increase in sample-processing throughput with the Echo system due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addi...

Next Generation Sequencing (NGS) has been a powerful tool to investigate gene networks in biological sciences [1]. Visualisation of data produced by NGS is essential for the interpretation of the findings by biological scientists. Here we describe a workflow to image findings from a NGS sequencing methodology to investigate gene expression that can be visualised with Circus software [2]. Visual...

2 In this work, we characterized the metagenome of a Malaysian mangrove soil sample via next 3 generation sequencing (NGS). Shotgun NGS data analysis revealed high diversity of microbes 4 from Bacteria and Archaea domains. The metabolic potential of the metagenome was 5 reconstructed using the NGS data and the SEED classification in MEGAN shows abundance of 6 virulence factor genes, implying th...

In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we d...