A total of 2,410 community residents aged 55 years and older were recruited from the Ningxia province of China. Apolipoprotein E (APOE) gene polymorphisms were detected using the high-resolution melting curve method. The Mini-Mental State Exam and Duke University Religion Index were administered to assess cognitive function and religiosity. The logistic regression model was used to examine the ...

Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

A rapid, duplex, high-resolution melting interleukin-28B gene (IL28B) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, fiv...