3T3 cells are shown to reduce SV40-3T3 cell population growth in a denisty-dependent manner in co-cultures of 3T3 and SV40-3T3 cells. The development of this inhibitory activity roughly parallels the development of density-dependent inhibition of growth in homogeneous 3T3 control cultures. The extent of reduction of SV40-3T3 growth can be manipulated by pretreatment of 3T3 cells with a high ser...

to the operation the patient is too much meddled with. After the usual dark-room examination the pa'.ient is put to bed, given a purge, the eye lashes and eye-briow shaved, an antiseptic dressing applied the night before operation. A weak solution of cocaine is used for the operation and several drops are put in the eye at intervals of ten mfinutes before the operation. The patient .is then bro...

T lymphocyte effectors to syngeneic SV40-transformed cells, generated by secondary in vitro sensitization of immune spleen cells, lyse SV40 transformed targets that are syngeneic at the H-2 locus. In this study we have employed recombinants within the H-2 region to examine in detail this H-2 specificity. H-2b effectors were found to lyse SV40-transformed targets from recombinants bearing either...

The regions of the simian virus 40 (SV40) core origin that are required for stable assembly of virally encoded T antigen (T-ag) and the T-ag origin binding domain (T-ag-obd(131-260)) have been determined. Binding of the purified T-ag-obd(131-260) is mediated by interactions with the central region of the core origin, site II. In contrast, T-ag binding and hexamer assembly requires a larger regi...

To better define protein-DNA interactions at a eukaryotic origin, the domain of simian virus 40 (SV40) large T antigen that specifically interacts with the SV40 origin has been purified and its binding to DNA has been characterized. Evidence is presented that the affinity of the purified T antigen DNA-binding domain for the SV40 origin is comparable to that of the full-length T antigen. Further...

Lombardo, Yolanda B., Cynthia Serdikoff, Manikkavasagar Thamotharan, Harbhajan S. Paul, and Siamak A. Adibi. Inverse alterations of BCKA dehydrogenase activity in cardiac and skeletal muscles of diabetic rats. Am. J. Physiol. 277 (Endocrinol. Metab. 40): E685–E692, 1999.— Rat cardiac and skeletal muscles, which have been used as model tissues for studies of regulation of branched-chain a-keto a...

JOCELYN DUPUIS,1 ANDREAS J. SCHWAB,2 ANDRÉ SIMARD,2 PETER CERNACEK,1 DUNCAN J. STEWART,3 AND CARL A. GORESKY†,2,4,5 1Department of Medicine, Montreal Heart Institute, Montreal H1T 1C8, 2McGill University Medical Clinic in the Montreal General Hospital, Departments of 4Medicine and 5Physiology, McGill University, Montreal, Quebec H3G 1A4, and 3Department of Medicine, St. Michael’s Hospital, Toro...

Murgia, Chiara, Isabella Vespignani, Joanna Cerase, Fabio Nobili, and Giuditta Perozzi. Cloning, expression, and vesicular localization of zinc transporter Dri 27/ZnT4 in intestinal tissue and cells. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G1231–G1239, 1999.—We have identified the Dri 27 cDNA on the basis of its upregulated expression during rat intestinal development. It encodes...

B. C. BERGMAN,1 G. E. BUTTERFIELD,2 E. E. WOLFEL,3 G. D. LOPASCHUK,4 G. A. CASAZZA,1 M. A. HORNING,1 AND G. A. BROOKS1 1Department of Integrative Biology, University of California, Berkeley 94720; 2Geriatric Research, Education, and Clinical Center, Palo Alto Veterans Affairs Health Care System, Palo Alto, California 95304; 3Division of Cardiology, University of Colorado Health Sciences Center,...

Aliaga, José Cristobal, Claude Deschênes, JeanFrançois Beaulieu, Ezéquiel L. Calvo, and Nathalie Rivard. Requirement of the MAP kinase cascade for cell cycle progression and differentiation of human intestinal cells. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G631–G641, 1999.—The intracellular signaling pathways responsible for cell cycle arrest and establishment of differentiated c...