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Iris nevus is common: 6% of patients with suspected iris melanoma have lesions other than melanoma, and 36% of them are nevi. Iris nevus turns into melanoma in approximately 8% of cases at a mean of 15 years. This case report provides the first description of an iris tumor examined with iris optical coherence tomography angiography (OCTA) compared to iris fluorescein angiography (IFA). A 60-yea...
Myelin oligodendrocyte glycoprotein (MOG) is commonly used as an immunogen to induce an immune response against endogenous myelin, thereby modeling multiple sclerosis in rodents. When MOG is combined with complete Freund's adjuvant (CFA), multifocal, multiphasic disease ensues; whereas when MOG is combined with incomplete Freund's adjuvant (IFA), clinical disease is usually absent. MOG-IFA immu...
Although mineral oils are generally considered nontoxic and have a long history of use in humans, the mineral oil Bayol F (incomplete Freund's adjuvant, IFA) and certain mineral oil components (squalene and n-hexadecane) induce lupus-related anti-nRNP/Sm or -Su autoantibodies in nonautoimmune mice. In the present study, we investigated whether medicinal mineral oils can induce other types of au...
Toxoplasmosis is a zoonosis of broad geographic distribution. This disease is caused by infection with the protozoan parasite Toxoplasma gondii. The most important ways to diagnose the disease are the serological techniques such as IFA and ELISA. The aim of this consideration, was comparison between the two methods, IFA and ELISA, in order to determine the incidence of antibody against Toxoplas...
Introduction: Detection of Cryptosporidium and Giardia parasites in water samples is usually performed by US Environmental Protection Agency 1623 method. Nevertheless, the USEPA1623 method still need improvement, to prevent and control the water borne parasitic disease. Therefore, we undertook the present study. Materials and methods: Totally 48 surface water samples were collected. Four sampl...
Objective(s): Brucellosis is a common infectious disease among animals and humans. While subunit vaccines could be used as an efficient strategy against pathogens, they usually seem to be less immunogenic than live or killed vaccines. However, the use of a suitable adjuvant accompanied by subunit vaccines can be a good alternative to enhance the immune response.Materials and Methods: To find a ...
An immunoglobulin M (IgM)-immunosorbent agglutination assay (IgM-IS-AGA) was negative in all sera from individuals negative in the Sabin-Feldman dye test, in sera from individuals with chronic Toxoplasma infection, and in cord blood samples from uninfected infants. In contrast, all sera that were obtained from individuals with a recent history of acute Toxoplasma infection and from infants with...
Purpose Detection of antinuclear antibodies has traditionally been performed by immunofluorescence (IFA) on fixed culture cells or tissues with reactivity being reported as a fluorescence pattern associated to one or more nuclear antigens. For this study, we compared results from IFA testing with a multiplex antinuclear antibody assay on a panel of patient serum samples previously evaluated by ...
We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid and sensitive detection of coxsackievirus A2, -4, -5, -6, and -10. This IFA kit was determined to have 95.9 to 100% sensitivity and 95.8 to 97.2% specificity. It also proved to be beneficial in reducing the number of enteroviruses that are untypeable in the clinical virology laboratory.
Serologically negative horses, as determined with the indirect fluorescent antibody test (IFA), were infected with Babesia equi and 60 days later with Babesia caballi. The only clinical signs of disease observed in these animals were a febrile reaction and slight icterus. Haematological changes included a drop in haematocrit and haemoglobin concentration, as well as lowered platelet counts. The...
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