Isoacceptors of Ala-, Arg-, Glu-, Gln-, Ile-, Leu-, Lys-, Ser-, Thr- and Val-tRNAs from wheat germ have been resolved by reverse phast chromatography. Codon recognition properties have been determined on isolated fractions of each of these aa-tRNAs and codon assignments have been made to a number of isoacceptors. Evolutionary changes which have occurred in patterns of codon recognition by isoac...

Cancer cells have altered metabolism compared to normal cells, including dependence on glutamine (GLN) for survival, known as GLN addiction. However, some cancer cell lines do not require GLN for survival and the basis for this discrepancy is not well understood. GLN is a precursor for antioxidants such as glutathione (GSH) and NADPH, and GLN deprivation is therefore predicted to deplete antiox...

Mutants of lactose permease of Escherichia coli with amino acid changes (Gly-24----Glu; Gly-24----Arg; Pro-28---Ser; Gly-24, Pro-28----Glu-Ser and Gly-24, Pro-28----Arg-Ser) within a putative membrane-spanning alpha-helix (Phe-Gly-Leu-Phe-Phe-Phe-Phe-Tyr-Phe-Phe-Ile-Met-Gly- Ala-Tyr-Phe-Pro-Phe-Phe-Pro-Ile) are incorporated into the cytoplasmic membrane. The mutant proteins retain the ability t...

Amino acid sequences of three canine bombesin-like peptides were determined after sequential purification of an extract obtained from 820 g of intestinal muscle. These three peptides contained 27, 23, and 10 amino acid residues. The sequences of the two shorter forms were identical to the corresponding carboxyl-terminal sequence of the heptacosapeptide. The sequence of the largest peptide is H2...

The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-BglII 3.6-kbp fragment of the plasmid pAM351(pPdl::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced pro...

Nogo-A protein consists of two main extracellular domains: Nogo-66 (rat amino acid [aa] 1019–1083) and Nogo-A-?20 (extracellular, active 180 region), which serve as strong inhibitors axon regeneration in the adult CNS (Central Nervous System). Although receptors S1PR2 HSPGs have been identified binding proteins, it remains at present elusive whether other directly interacting with exist, decrea...

Substance P (SP: Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-GlyLeu-Met-NH2), neurokinin A (NKA: His-Lys-Thr-Asp-ThrPhe-Val-Gly-Leu-Met-NH2) and neurokinin B (NKB: AspMet-His-Asp-Phe-Phe-Val-Gly-Leu-Met-NH2) are mammalian tachykinin peptides that have similar biological actions, such as smooth muscle contraction, salivation, vasodilatation and neuronal excitation. These mammalian tachykinin peptides are ch...

Several research groups have identified IgE-binding wheat proteins associated with baker's asthma or atopic dermatitis. This review pinpoints our attempts at the structural identification of wheat allergens using sera from atopic dermatitis patients with definite wheat allergy. Two thirds of patients tested were gluten-sensitive. An IgE-binding peptide was isolated from the chymotryptic hydroly...

Two new cyclic lipopeptides maribasins A (1) and B (2) were isolated from the fermentation broth of the marine microorganism Bacillus marinus B-9987 isolated from Suaeda salsa in Bohai coastline of P. R. China. Both structures were established to be cyclo (D-Pro-L-Gln-L-Asn-L-Ser-D-Asn¹-D-Tyr-D-Asn²-D-β-aminoisopentadecanoic acid) (1) and cyclo (D-Pro-L-Gln-L-Asn-L-Ser-D-Asn¹-D-Tyr-D-Asn²-D-β-a...