A novel, fast and sensitive determination strategy for E. coli O157:H7 has been developed by combination of ligandmagnetic nanoparticles (LMNPs) enrichment with a fluorescent silica nanoparticles (FSiNPs) based two-color flow cytometry assay (LMNPs@FSiNPs-FCM). E. coli O157:H7 was first captured and enriched through the lectin concanavalin A (Con A) favored strong adhesion of E. coli O157:H7 to...

Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndrome in humans. EHEC O157:H7 strains are not pathogenic in calves >3 weeks old. Our objective was to determine if EHEC O157:H7 strains are pathogenic in neonatal calves. Calves <36 h old inoculated with ...

Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic...

In this study, the survival of Escherichia coli O157:H7 and non-O157 STEC serogroups O26, O111, O103, O145 were investigated during production storage yogurt. For purpose, pathogens individually inoculated into milk after pasteurization along with starter culture (approximately 7.00±1.00 log10 cfu/g). After incubation at 44oC (about 180 min), yogurt samples capped stored 4oC for 20 days. Pathog...

The present study aimed to evaluate the antibacterial effect and antioxidant potential of quercetin nanoparticles on drug-resistant E. coli O157 in New Zealand White Rabbits. Initial surveillance lasting two years (2018-2019) was performed isolate molecular detection some virulence genes drug-resistant E.coli strains circulating NZW rabbits Egypt.The selected E.coli O157 strain our experimental...

Studies were conducted to evaluate fecal shedding of Escherichia coli O157:H7 in a small group of inoculated deer, determine the prevalence of the bacterium in free-ranging white-tailed deer, and elucidate relationships between E. coli O157:H7 in wild deer and domestic cattle at the same site. Six young, white-tailed deer were orally administered 10(8) CFU of E. coli O157:H7. Inoculated deer we...

Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd(F)) located in the chromosomal backbone of the pathogenic O157:H7 Escherichia coli strain (ccd(O157)). The ccd(F) and the ccd(O157) systems coexis...

BACKGROUND Escherichia coli serogroup O157:H7 has emerged as an important zoonotic bacterial pathogen, causing a range of symptoms from self-limiting bloody diarrhea to severe hemorrhagic colitis and hemolytic-uremic syndrome in humans. Beef and dairy cattle are considered the most important animal reservoirs for this pathogen. One of the important virulence characteristics of E. coli O157:H7 i...

Objetivo. Evaluar la calidad higiénico-sanitaria de leche bovina Nariño mediante los recuentos mesófilos aerobios y células somáticas determinar presencia microorganismos ambientales importantes en salud pública como Salmonella spp., Escherichia coli O157, Listeria monocytogenes, Staphylococcus aureus Yersinia enterocolítica evaluar las variaciones parámetros dos periodos lluviosos diferentes. ...

Aims The purpose of this study was to investigate the occurrence Escherichia coli O157 and O26 on Belgian dairy cattle farms, presence virulence genes in confirmed isolates association E. with calf diarrhoea. Methods Results In total, 233 recto-anal mucosal swabs (RAMS) were obtained from healthy diarrheic calves three each alternately visited consecutive times. RAMS analysed for O26, stx1, stx...