With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...

background and objectives: shiga toxin-producing escherichia coli (stec) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. we compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of escherichia coli isolated from feces of diarrheatic calves in tehran suburb farms. materials a...

the aim study antibacterial effects of silver nanoparticles against resistant strains of e.coli bacteria.12 strains of e. coli strains arising urinary infection were isolated from hospitalized patient in zabolhospitals. minimum inhibitory concentration of winter cherry were determined by dilution method in various concentration on bacteria. the highest mic values was found to be 100ppm against ...

results the results showed that the isolated e. coli strains were sensitive to these antibiotics: erythromycin (52.94%), tetracycline (76.47%), ceftazidime (41.17%), cefixime (35.29%), penicillin (76.47%), ampicillin (58.82%) and nalidixic acid (41.17%). examination of the herbal extracts showed that the highest maximum inhibitory concentration (mic) against drug resistant e. coli was 200 ppm. ...

In modern medical processes, new antimicrobial drugs are being widely used in order to rapid and essential eradication of resistant bacterial strains. Broad-spectrum beta-lactamases are enzymes originated from mutations in the common plasmid genes mediators for beta-lactamases such as TEM-1, TEM-2, and SHV-1 are transferred among bacterial species. The genes coding the production of ESBL are of...

conclusions anova results showed that the effect of cycle and time on the selected bacterial removal efficiency were more important than that of the microbial concentration. results the results showed that e. coli and e. faecalis were effectively treated at 10 pulse/s in 9 minutes and 6 log cfu/ml bacterial suspension (p < 0.0001 for e. coli with 99.99% (4 log) and p = 0.0002 for e. faecalis wi...

conclusions the alcoholic extract of p. harmala was very effective against the selected esbl-producing e. coli isolates harboring the tem gene. therefore, it could be suggested as an antibacterial agent in the future. more researches are necessary for detecting the mechanism of this plant’s behavior and its pharmacological effects. results results of this study showed that in disk diffusion met...