نتایج جستجو برای: آمیلوراید amiloride

تعداد نتایج: 2783  

2009
Konstantin Pervushin Edward Tan Krupakar Parthasarathy Xin Lin Feng Li Jiang Dejie Yu Ardcharaporn Vararattanavech Tuck Wah Soong Ding Xiang Liu Jaume Torres

The envelope (E) protein from coronaviruses is a small polypeptide that contains at least one alpha-helical transmembrane domain. Absence, or inactivation, of E protein results in attenuated viruses, due to alterations in either virion morphology or tropism. Apart from its morphogenetic properties, protein E has been reported to have membrane permeabilizing activity. Further, the drug hexamethy...

Journal: :Journal of virology 2008
David N Harrison Elena V Gazina Damian F Purcell David A Anderson Steven Petrou

Amiloride derivatives are known blockers of the cellular Na(+)/H(+) exchanger and the epithelial Na(+) channel. More recent studies demonstrate that they also inhibit ion channels formed by a number of viral proteins. We previously reported that 5-(N-ethyl-N-isopropyl)amiloride (EIPA) modestly inhibits intracellular replication and, to a larger extent, release of human rhinovirus 2 (HRV2) (E. V...

Journal: :The American journal of physiology 1999
Peter Steen Pedersen Niels-Henrik Holstein-Rathlou Per Leganger Larsen Klaus Qvortrup Ole Frederiksen

Airway epithelium explants from cystic fibrosis (CF) patients and non-CF subjects formed monolayered spheres, with the apical ciliated cell membrane facing the bath and the basolateral cell membrane pointing toward a fluid-filled lumen. With the use of two microelectrodes, transepithelial potential difference and changes in potential difference in response to passage of current pulses were reco...

Journal: :Journal of neurophysiology 2016
Devaki Kumarhia Lianying He Lynnette Phillips McCluskey

Inflammation-mediated changes in taste perception can affect health outcomes in patients, but little is known about the underlying mechanisms. In the present work, we hypothesized that proinflammatory cytokines directly modulate Na(+) transport in taste buds. To test this, we measured acute changes in Na(+) flux in polarized fungiform taste buds loaded with a Na(+) indicator dye. IL-1β elicited...

Journal: :The Journal of General Physiology 1997
Ming Lu Gerhard Giebisch WenHui Wang

We have used the patch clamp technique to study the effects of inhibiting the apical Na+ transport on the basolateral small-conductance K+ channel (SK) in cell-attached patches in cortical collecting duct (CCD) of the rat kidney. Application of 50 microM amiloride decreased the activity of SK, defined as nPo (a product of channel open probability and channel number), to 61% of the control value...

Journal: :Eukaryotic cell 2005
Jürgen Stolz Heike J P Wöhrmann Christian Vogl

Amiloride, a diuretic drug that acts by inhibition of various sodium transporters, is toxic to the fission yeast Schizosaccharomyces pombe. Previous work has established that amiloride sensitivity is caused by expression of car1+, which encodes a protein with similarity to plasma membrane drug/proton antiporters from the multidrug resistance family. Here we isolated car1+ by complementation of ...

Journal: :The Journal of biological chemistry 1992
Y Oh S Matalon T R Kleyman D J Benos

An amiloride binding protein in adult rat and rabbit alveolar type II (ATII) cells was characterized using three different antibodies against epithelial Na+ channel proteins. We found that 1) polyclonal antibodies raised against epithelial Na+ channel proteins from bovine kidney cross-react with a 135-kDa protein in ATII membrane vesicles on Western blots; 2) using the photoreactive amiloride a...

Journal: :American journal of physiology. Renal physiology 2002
Gustavo Frindt Tiffany McNair Anke Dahlmann Emily Jacobs-Palmer Lawrence G Palmer

To test the role of epithelial Na channels in the day-to-day regulation of renal Na excretion, rats were infused via osmotic minipumps with the Na channel blocker amiloride at rates that achieved drug concentrations of 2-5 microM in the lumen of the distal nephron. Daily Na excretion rates were unchanged, although amiloride-treated animals tended to excrete more Na in the afternoon and less in ...

2013
Eok-Cheon Kim Soo-Kyoung Choi Mihwa Lim Soo-In Yeon Young-Ho Lee

AIMS Mechanogated ion channels are predicted to mediate pressure-induced myogenic vasoconstriction in small resistance arteries. Recent findings have indicated that transient receptor potential (TRP) channels and epithelial sodium channels (ENaC) are involved in mechanotransduction. The purpose of this study was to investigate the role of TRP channels and ENaC in the myogenic response. Our prev...

Journal: :The Journal of General Physiology 1982
C W Davis A L Finn

In toad urinary bladder epithelium, inhibition of Na transport with amiloride causes a decrease in the apical (Vmc) and basolateral (Vcs) membrane potentials. In addition to increasing apical membrane resistance (Ra), amiloride also causes an increase in basolateral membrane resistance (Rb), with a time course such that Ra/Rb does not change for 1-2 min. At longer times after amiloride (3-4 min...

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