Many applications have been found for site-specific recombinases (SSRs) in DNA and genome engineering. Cre recombinase from the coliphage P1 and FLP recombinase from the Saccharomyces cerevisiae 2 micron circle have become the most widely used SSRs (Branda and Dymecki, 2004; Glaser et al., 2005). Both belong to the class of tyrosine recombinases because they share a common reaction mechanism, i...

Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmon...

susceptibility maps obtained with the CF solution (Rho = 0.94, r = 0.97) on the regions of interest, GP -globus pallidus, SN susbtantia nigra, C caudate, RN red nucleus, P putamen, FM forceps major, IFOF inferior fronto-occipital fasciculus Fig. 1 shows error of QSM maps in respect to Ground Truth (zero being lowest reconstruction error) as a function of the regularization parameters λ (see Eq....

We present ⦇λ⦈, a calculus with special constructions for dealing effects and handlers. This is an extension of the simply-typed λ-calculus (STLC). enrich STLC type representing effectful computations alongside operations to create process values this type. The motivated by natural language modelling, especially semantic representation. Traditionally, meaning sentence calculated using λ-terms, ...

Suppose G is an nvertex and medge simple graph with edge set E(G). An integervalued function f: E(G) → Z is called a flow. Tutte was introduced the flow polynomial F(G, λ) as a polynomial in an indeterminate λ with integer coefficients by F(G,λ) In this paper the Flow polynomial of some dendrimers are computed.

Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including thei...