Clinical and microbiological follow-up of an outbreak of Yersinia Pseudo-tuberculosis serotype 1b. Scand J Infect Dis.logical features of Yersinia infection in a territory with developed agricultural production. A widespread outbreak of Yersinia pseudotuber-culosis O:3 infection from iceberg lettuce. outbreak of gastrointestinal illness and erythema nodosum from grated carrots contaminated with...

Pathogenic Yersinia spp. utilize a plasmid-encoded type III secretion system to deliver a set of Yop effector proteins into eukaryotic cells. Previous studies have shown that the effector YopJ is required for Yersinia to cause downregulation of the mitogen-activated protein (MAP) kinases c-Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK) 1 and 2 in infected macr...

YopB is a 401-amino-acid protein that is secreted by a plasmid-encoded type III secretion system in pathogenic Yersinia species. YopB is required for Yersinia spp. to translocate across the host plasma membrane a set of secreted effector proteins that function to counteract immune signaling responses and to induce apoptosis. YopB contains two predicted transmembrane helices (residues 166 to 188...

Pathogenic Yersinia spp. carry a large common plasmid that encodes a number of essential virulence determinants. Included in these factors are the Yersinia-secreted proteins called Yops. We analyzed the consequences of wild-type and mutant strains of Yersinia pseudotuberculosis interactions with the macrophage cell line RAW264. 7 and murine bone marrow-derived macrophages. Wild-type Y. pseudotu...

Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examined, and their parents were interviewed concerning symptoms. Rotavirus, adenovi...

The plasmid-encoded type three secretion system (TTSS) of Yersinia spp. is responsible for the delivery of effector proteins into cells of the innate immune system, where these effectors disrupt the target cells' activity. Successful translocation of effectors into mammalian cells requires Yersinia to both insert a translocon into the host cell membrane and sense contact with host cells. To pro...

A comparison was made of the in vitro activities of norfloxacin and of nine other orally administered antibacterial agents against 180 clinical isolates representing the bacterial species most frequently implicated in infections of the gastrointestinal tract in humans. The 90% minimal inhibitory concentrations showed norfloxacin to be 4, 15, 4, 17, 17, 17, and 33 times more active than the next...

A simple, rapid, field-adapted indirect enzyme-linked immunoassay (FldELISA) for the detection of antibodies to Brucella abortus in whole blood and serum has been developed. This assay detects antibodies to B. abortus in approximately 15 min or less. Over a 3-month period, this assay has consistently identified immunized and nonimmunized animals, while the percent coefficient of variation for e...

A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigel...

Natural honey is an ancient remedy in folk medicine around the world particularly where conventional and modern therapeutic agents fail. Here we report antibacterial potential of a number of unifloral and multifloral natural honey samples produced in Pakistan. Honey samples were collected from colonies of Apis mallifera and Apis cerana bees foraged on Acacia modesta, Plactranthus speices, Zizip...