Histone deposition proteins CAF-1 and Asf-1 impact multiple aspects of chromosome formation and are essential in yeast to prevent DNA damage and avoid chromosomal rearrangement. Using fractionated yeast cell extracts, this MQP attempted to identify stimulatory factors which are necessary and sufficient for histone deposition by CAF-1 and/or Asf1. Identification of these cofactors, would provide...

Aspergillus ochraceus NRRL 3174 produced 29 mg of ochratoxin A per 100 ml of nutrient medium consisting of 4% sucrose and 2% yeast extract. Ochratoxin A was the sole metabolite present in the chloroform extracts of the growth medium. Trace amounts of ochratoxin B were produced in a 1% yeast medium, and a comparatively large amount of ochratoxin B was produced in media containing 16 and 32% sucr...

Objective: Punica granatum, commonly known as pomegranate, has emerged as a medicinal plant with potential antimicrobial activity. The present study was planned to evaluate this activity against both Gram positive Staphylococcus aureus (S. aureus) and negative Pseudomonas aeruginosa (P. aeruginosa) bacteria as well as against pathogenic yeast, Candida albicans (C. albicans). Material and Metho...

Thiobacillus novellus was able to grow with oxalate, formate, formamide, and methanol as sole sources of carbon and energy. Extensive growth on methanol required yeast extract or vitamins. Glyoxylate carboligase was detected in extracts of oxalate-grown cells. Ribulose bisphosphate carboxylase was found in extracts of cells grown on formate, formamide, and thiosulfate. These data indicate that ...

Methanolic extracts of flowers and leaves of Gentiana lutea L., together with the isolated compounds mangiferin, isogentisin and gentiopicrin, were used to investigate the antimicrobial activity of the plant. A variety of Gram-positive and Gram-negative bacteria as well as the yeast Candida albicans has been included in this study. Both extracts and isolated compounds showed antimicrobial activ...

The ethanol and water extracts of Sansevieria trifasciata leaves showed dose-dependent and significant (P < 0.05) increase in pain threshold in tail-immersion test. Moreover, both the extracts (100 - 200 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition of both phases of the formalin pain test. The ethanol extract (200 mg/kg) significa...

In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to pol...

DNA coding for 234 amino acids of the circumsporozoite (CS) protein of Plasmodium vivax was incorporated into yeast expression vectors. The DNA encoded all the repeat domain and codons for a highly conserved sequence, KLKQP, found in CS proteins from all malaria parasites. Yeast cells transformed with these autonomously replicating plasmids expressed, upon induction, high levels of the CS polyp...

This study we have conducted an ethnobotanical survey and antimicrobial activity in Tetouan province to identify the use of famous plant species traditional medicine : fig tree Olive follow-up during three stages for green, pink black olives mixing olive extracts. 80 questionnaire sheets were done April-June 2019, method diffusion was used activity.Bacteria yeasts used: Staphylococcus aureus, P...