In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, usin...

Since the introduction of our plasma cortisol enzyme-linked immunosorbent assay (ELISA) [l] using affinity purified antiserum [2] into the routine steroid laboratory, we have embarked on the development of a simple urinary free or unconjugated cortisol ELISA which would allow measurement of both urine and plasma cortisol on the one ELISA plate. This paper describes for the first time an ELISA f...

BACKGROUND Kagera is one of the 22 regions of Tanzania mainland, which has witnessed a decline in HIV prevalence during the past two decades; decreasing from 24% in 1987 to 4.7 in 2009 in the urban district of Bukoba. Access to social capital, both structural and cognitive, might have played a role in this development. The aim was to examine the association between individual structural and cog...

TO THE EDITOR—Jarvis et al describe the evaluation of a point-of-care antigen test for cryptococcal meningitis against a quantitative sandwich enzyme-linked immunosorbent assay using the Spear-man rank correlation coefficient [1].

Enzyme-linked immunosorbent assays (ELISA) for detecting malarial parasites in blood were tested on uninfected monkeys and in monkeys infected with Plasmodium falciparum. A double antibody sandwich ELISA detected one malarial cell per 10(3) uninfected cells, and an inhibition ELISA detected on infected cell per 10(4) uninfected erythrocytes. These methods are not yet as sensitive as conventiona...

The aim of this study was to examine the validity and reliability of a novel immunoassay, developed to assess salivary Immunoglobulin A (s-IgA). Validity and reliability of the Individual Profiling Lateral Flow Device (IPRO LFD) for s-IgA concentrations ([s-IgA]) was assessed in males (n = 12) and females (n =13) who were involved in recreational activities. Reliability of the IPRO LFD method w...

A competitive and a sandwich enzyme linked immunosorbent assay (ELISA) were developed for human leukocyte collagenase and gelatinase. The competitive assay could detect 0.5 ng collagenase and 0.05 ng gelatinase. The detection limit of the sandwich ELISA was 0.05 ng for collagenase and 0.02 ng for gelatinase. No cross reactivity between human leukocyte collagenase and gelatinase was detected. Th...

OBJECTIVE To describe the clinical, hematological and radiographic characteristics of children hospitalized for Mycoplasma pneumoniae pneumonia. METHOD The study population consisted of 190 children between 3 months and 16 years old, hospitalized for radiographically confirmed pneumonia. Patients were divided into two groups, to wit: 95 children with Mycoplasma pneumoniae pneumonia, as diagno...

An enzyme-linked immunosorbent assay (ELISA) test has been developed to detect antibodies to Legionella pneumophila serogroup 1. There is good correlation between indirect fluorescent antibody (IFA) and ELISA titres but ELISA is more sensitive.

Serum C-reactive protein (CRP) concentrations in healthy beagle dogs of various ages and in pregnant beagles were measured by enzyme-linked immunosorbent assay (ELISA). Serum CRP concentrations were 1.5-16.0 microg/ml (mean 7.9 +/- 3.4 microg/ml) in male, and 1.8-18.9 microg/ml (mean 8.3 +/- 4.0 microg/ml) in female dogs. No significant sex-related differences were observed in the values. Furth...