The nuclease activity of 1,10-phenanthroline-copper ion can be targeted to specific DNA sequences by attachment of the ligand to the 5' end of complementary deoxyoligonucleotides via a phosphoramidate linkage. To synthesize the adduct, the phosphorimidazolide of the deoxyoligonucleotide is prepared using a water-soluble carbodiimide and is then coupled to 5-glycylamido-1,10-phenanthroline. Afte...

The present study shows that the sulfur of the phosphorothioate 5'-nucleoside diphosphate beta-S adds across the olefinic double bond of the maleimide moiety of the thiol-sensitive probe, N-l pyrene maleimide. The reaction of phosphorothioate with the maleimide moiety provided a basis for coupling the phosphorothioate nucleoside diphosphate beta-S to maleimidederivatized bovine serum albumin. F...

Synthesis, structure determination, and magnetic properties are reported for the metastable and crystal-chemically isotypic phases cobalt carbodiimide, CoNCN, and nickel carbodiimide, NiNCN, adopting the hexagonal system and space group P63/mmc (NiAs type) with interatomic distances of Co-N = 2.17 Angstrom and Ni-N = 2.12 Angstrom and an octahedral coordination of the transition-metal ions; the...

Specific and strong cellulose-binding characteristics were utilized for promoting retention of additives in contaminated papermaking systems. Cellulose-binding domain (CBD) of cellulase derived from Trichoderma viride was separated by digestion with papain, and then introduced into anionic polyacrylamide (A-PAM) through a condensation reaction using water-soluble carbodiimide. The CBD-modified ...

A 42-kDa bovine protein that binds bovine angiogenin [angiogenin binding protein (AngBP)] has been identified as a dissociable cell-surface component of calf pulmonary artery endothelial cells and a transformed bovine endothelial cell line, GM7373. Binding of 125I-labeled bovine angiogenin (125I-Ang) to AngBP occurs with an apparent Kd approximately 5 x 10(-10) M and is specific, saturable, and...

A conserved actin-binding domain (Mr = 27,000) of rat hepatic actinogelin, rat skeletal muscle, and chicken gizzard alpha-actinins (Mimura, N., and Asano, A. (1986) J. Biol. Chem. 261, 10680-10687) was separated into two components having different isoelectric points (peptides A and B) by chromatofocusing. Thermolysin digestion of peptide A generated peptide B with concomitant loss of peptide A...

Since a basic surface on the catalytic (C) subunit of cAMP-dependent protein kinase is important for binding to the regulatory (R) subunit, acidic residues in R were sought that might contribute to R-C interaction. Using differential labeling by a water-soluble carbodiimide (Buechler, T. A., and Taylor, S. S. (1990) Biochemistry 29, 1937-1943), seven specific carboxylates in RIalpha were identi...

Bicomponent electrospun nanofibers based on the combination of synthetic (i.e., aliphatic polyesters such as polycaprolactone (PCL)) and natural proteins (i.e., gelatin) have been extensively investigated as temporary platforms to instruct cells by the release of molecular/pharmaceutical signals for the regeneration of several tissues. Here, water soluble proteins (i.e., gelatin), strictly embe...

An improved direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the analysis of cyclopiazonic acid (CPA) in corn, peanuts, and mixed feed. Two new approaches were used for the preparation of enzyme markers; one involved coupling CPA-bovine serum albumin (CPA-BSA) conjugate to horseradish peroxidase (HRP) using either the glutaraldehyde (GA) or the periodate (PI) me...

Covalent grafting of biomolecules is a strategy to improve the biocompatibility and bioactivity of materials. However, it is critical to maintain the biological activity of the biomolecule upon its attachment to the surface. In the present study we compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobiliz...