نتایج جستجو برای: was not detected reverse transcription
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To investigate the distribution of tumour cells expressing the SYT-SSX fusion gene in biphasic synovial sarcoma, modified reverse transcription polymerase chain reaction (RT-PCR) analysis was performed using microdissected specimens from haematoxylin and eosin stained sections of archival paraffin wax embedded tissues. This modified RT-PCR included a stage with degenerate oligonucleotide primed...
Survey of Sars-Cov-2 Genetic Material Reduction During a Traditional Wastewater Treatment Technology
The transmission of Severe Acute Respiratory Syndrome Coronavirus-2 in a community can be monitored by wastewater-based epidemiological approach due to fecal shedding. Although sewage surveillance has gained considerable amount attention over the last 16 months, an indirect issue within topic is whether traditional wastewater treatment technologies are sufficiently efficient eliminate genetic m...
Here we provide the first strategy to use a competitive Extendable Blocking Probe (ExBP) for allele-specific priming with superior selectivity at the stage of reverse transcription. In order to analyze highly similar RNA variants, a reverse-transcriptase primer whose sequence matches a specific variant selectively primes only that variant, whereas mismatch priming to the alternative variant is ...
Background Persistent human bocavirus 1 (HBoV1) infection is a common finding in patients suffering from chronic tonsillar disease. However, the associations between HBoV1 and specific immune reactions are not completely known. We aimed to compare vivo expression of T-cell cytokines, transcription factors, type I/III interferons tonsils HBoV1-positive -negative tonsillectomy patients. Methods T...
Since 2019, SARS-COV2 has become a global pandemic. Many hospitals designated hostilas for the treatment of patients. As part preventive measures, are required to conduct routine desinfection procedure minimize risk virus transmission. Therefore this study aims evaluate surface contamination SARS COV2 at emergency ward, outpatients ward and inpatient in how hospital. Surface samples were collec...
esl/efl books play a crucial role in shaping language learners worldview of gender roles in society. the present study investigated the status of sexism in two sets of efl textbooks, one developed by non-native iranian authors (ili series) and the other by native authors (top notch series). first, two books from each series was selected randomly. then, a quantitative analysis was carried out wi...
building on previous studies on intellectual features and learners’ grammar learning, the present study aimed at investigating whether intelligence criterion had any impact on efl learners’ english grammar learning across two intelligence levels. in the current study, the participants were divided into two experimental and control groups by administration of raven i.q. test. this led to the for...
126 abstract: in this research we studied the effects of cyclic hydration and dehydration of cysts of artemia urmiana and artemia parthenogenetica on the hatching percentage, survival and growth. the experiment was carried out in 3 treatments (1-3 hydration/dehydration cycles) with 3 replicates for each treatment. later effects of cold preservation at -20°c during three time periods were ...
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