نتایج جستجو برای: was amplified and sequenced using conventional rt
تعداد نتایج: 17375690 فیلتر نتایج به سال:
Supplemental Experimental Procedures Plasmids The N-terminal and C-terminal fragments of Claspin were separately amplified by RT-PCR using a cDNA library generated from HEK293T cells. The two RT-PCR products were sequentially inserted into pcDNA3.1-HA tag to obtain a plasmid containing full-length Claspin. Claspin mutants were generated using the QuickChange Site-directed Mutagenesis kit (Strat...
today, the route for economic development in most countries is the same as international open competitive economy. economic institutes well known that supportive public economy belonged on the past and they may compete in the global economy. it is obvious that if they have no competitive potency or not familiar with competition technique, they may be devastated. banking system aims to collect t...
بررسی تنوع ژنتیکی ژنوتیپهای بومی پیاز ایرانی در مقایسه با انواع خارجی چکیده: برنامه های اصلاحی گیاهان بر اساس تنوع و انتخاب صفات برتر کمی و کیفی صورت می گیرد. لذا، ارزیابی تنوع ژنتیکی، اولین مرحله در برنامه های اصلاحی است. در این راستا، استفاده از روش های جدید مطالعهی تنوع ژنتیکی ضروری به نظر می رسد. در این بررسی تعیین تنوع ژنتیکی سیزده ژنوتیپ پیاز ایرانی در مقایسه با دو ژنوتیپ خارجی با است...
Abstract During 2017–2018, nasopharyngeal aspirates (NPAs) from 627 hospitalized patients with severe acute respiratory infection at Luohe Center Hospital were tested by RT-PCR for human parainfluenza virus 4 (HPIV-4). Fourteen (2.2%) of the samples positive HPIV-4. The complete HN gene was amplified nine and sequenced. Sequence comparisons showed that HPIV-4 strains circulating in city are clo...
nitric oxide (no) is a small molecule synthesized by most of mammalian cells with diverse biological activities including vasodilatation, host defense and wound healing. impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. on the other hand the application of laser in biomedical area have been increased. thus, the current studies were desi...
this work is presented in five parts. in the first part preparation of the starting complex [pt(c^n)cl(dmso)], 1, in which c^n = n(1),c(2?)-chelated, deprotonated 2-phenylpyridine, and dmso = dimethylsulfoxide, and its reaction with 1 equiv of the biphosphine ligands bis(diphenylphosphino)amine, dppa, or bis(diphenylphosphino)methane, dppm, to give the complex [pt(c^n)cl(dppa)], 2, or [pt(c^n)c...
this study attempted to explore if teaching english collocations through two different modes of awareness-raising and input flooding has any possible differential effect on immediate retention as well as retention in a delayed assessment. it also compared the possible differential effect of teaching english collocations implicitly and explicitly on actively using the items in writing. m...
efl/esl textbooks have been regarded as essential language teaching materials with which the learners spend about 70 up to 90 percent of their class time. the important role they play and their vast use make them not only influential in learning the language but also in shaping values and attitudes. put it another way, textbooks socialize learners using their contents (i.e. texts, illustrations...
anxiety is one common disorder during childhood. in curing anxiety disorders, there are different types of psychological remedies, pharmotherapy, family therapy, and music therapy that can improve the individual’s anxiety, but we need some other remedies to improve social and emotional relationship and interactions. considering the importance of curing the anxiety disorders, the effect of sand ...
the debate whether esp teachers need to have knowledge of the specialized field they are teaching has been an issue in the literature over the last two decades. the aim of conducting this study was to explore iranian learners’ and instructors’ beliefs and attitudes concerning the role of specialized knowledge in teaching esp classes. a seventeen item questionnaire was administered to 400 esp le...
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