The final assembly of rotavirus particles takes place in the endoplasmic reticulum (ER). In this work, we evaluated by RNA interference the relevance to rotavirus assembly and infectivity of grp78, protein disulfide isomerase (PDI), grp94, calnexin, calreticulin, and ERp57, members of the two ER folding systems described herein. Silencing the expression of grp94 and Erp57 had no effect on rotav...

While only four globally important rotavirus G serotypes (1 to 4) have been documented, many studies suggest that serotype G9 viruses may be widely distributed and more important than previously recognized. We have evaluated 10 serotype G9 rotavirus-neutralizing monoclonal antibodies (MAbs) directed to VP7, which bound by direct enzyme immunoassay (EIA) to P1A[8], G9 rotaviruses F45, WI61, and ...

A binary classification system has been established for group A rotaviruses, with the viral capsid protein VP7 defining G types and VP4 defining P types. At least 15 G types and 21 P types have been isolated globally with various G and P combinations. Most of the currently circulating human rotaviruses belong to G1P[8], G2P[4], G3P[8], and G4P[8]. We report a human rotavirus strain (B1711) with...

Rotavirus A (RVA) is recognized as a major etiology responsible for the development of acute gastroenteritis in children worldwide. The purpose present study was to perform molecular characterization RVA. total 323 stool specimens collected from hospitalized with Chiang Rai, Thailand, 2016-2018 were identified G- and P-genotypes through RT-PCR analysis. RVA more prevalent 2017-2018 (37.8%) than...

A rotavirus sample collection from 19 consecutive years was used to investigate the heterogeneity and the dynamics of evolution of G1 rotavirus strains in a geographically defined population. Phylogenetic analysis of the VP7 gene sequences of G1P[8] human rotavirus strains showed the circulation of a heterogeneous population comprising three lineages and seven sublineages. Increases in the circ...

having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. in present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and rapd markers. significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. sheep are intermediate hosts of four sarcocystis species: sarcocystis tenella, sarcocystis gigantea, sarcocystis arieticanis, and sarcocystis medusiformision. the purpose of the study was to determine the molecular identification of the macroscopic and microscopic cysts of sarcocystis in sheep. in...