Crypt cells were also distinguished from vilus cells by their preferential agglutination with ConA3 (7). Human fetal intestinal cells also have plasma membrane galactosyltrans ferase and are agglutinated by ConA (13). In this study we have extended our observations to the neoplastic intestinal epithelial cell in both small intestine and colon of rats. The carcinogen DMH was used to induce colon...

The effects of feeding a nutritionally adequate liquid diet containing 5% ethanol to rats over a four week period on intestinal lactase activity and the kinetics of jejunal galactose absorption in vivo have been determined. Both lactase activity and the maximum capacity for active, saturable galactose absorption (Jmax) were increased significantly after chronic ethanol ingestion. In contrast, u...

The molecular identity of the apical HCO3(-)-secreting transporter in gastric mucous cells remains unknown despite its essential role in preventing injury and ulcer by gastric acid. Here we report the identification of a Cl-/HCO3- exchanger that is located on apical membranes of gastric surface epithelial cells. RT-PCR studies of mouse gastrointestinal tract mRNAs demonstrated that this transpo...

The objective of this study was to determine whether 2 distinct chlamydial isolates recovered from the intestines and feces of diarrheic nursery pigs could cause intestinal lesions in gnotobiotic pigs. Both isolates share biological characteristics with Chlamydia trachomatis. Chlamydial isolates R27 and R19 were propagated in Vero cells or embryonated eggs, respectively, and suspended in sucros...

Abstract The objective of this study was to investigate the effects in ovo injection sodium borate on hatching power, chick weight, length fertile broiler eggs. A total 256 eggs were incubated study. On Day 18 incubation, two groups injected with 0.1 mL 0.5 mg and 1 dissolved saline, used as sham control (injected saline) uninjected control. Hatching power apparently increased ( P ≤ 0.05) by (0...

Krüppel-like factor 9 (Klf9), a zinc-finger transcription factor, is implicated in the control of cell proliferation, cell differentiation, and cell fate. Using Klf9-null mutant mice, we have investigated the involvement of Klf9 in intestine crypt-villus cell renewal and lineage determination. We report the predominant expression of Klf9 gene in small and large intestine smooth muscle (muscular...

We describe the changes of several brush-border enzymatic activities in different subpopulations of epithelial cells, separated sequentially from the villus tip-to-crypt axis of the small intestine, induced by deprivation of dietary nucleotides for different periods of time in adult rats. Deprivation of dietary nucleotides lead to a decrease in the content and specific activity of alkaline phos...