The occurrence, diversity, and pathogenicity of Vibrio spp. were investigated in two estuaries along the Italian Adriatic coast. Vibrio alginolyticus was the predominant species, followed by Vibrio parahaemolyticus, non-O1 Vibrio cholerae, and Vibrio vulnificus. By using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. Th...

Vibrio parahaemolyticus cells are readily inactivated in distilled water. The time of exposure required to inactivate 90% of the cells was between 0.9 and 4.4 min.

BACKGROUND Recently isolated Vibrio parahaemolyticus strains have displayed multiple antibiotic resistance. Alternatives to conventional antibiotics are needed, especially for the multiple-antibiotic-resistant V. parahaemolyticus pandemic strain. METHODS A bacteriophage, designated pVp-1, showed effective infectivity for multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus, inc...

A recent outbreak of gastroenteritis caused by Vibrio parahaemolyticus following consumption South Australian oysters has brought to the forefront an increasing hazard for oyster industry and consumers. pathogens have been sparsely implicated in illnesses Australia; however, rising sea temperatures varied weather conditions delivered climate change could be driving increased prevalence these ou...

vibrio species are the major seafood-borne bacteria that are frequently associated with the consumption of contaminated sea food. a total of 113 samples including 58 fish and 55 shrimps were studied for the possible contamination with vibrio species. a biochemical protocol was applied for the identification of the vibrio isolates and polymerase chain reaction (pcr) was carried out to confirm th...

OBJECTIVES A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. METHODS Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus,...

Among the more than 70 different Vibrio species inhabiting marine, estuarine, and freshwater ecosystems, 12 are recognized as human pathogens. The warm subtropical climate of the Black Sea coastal area and inland regions of Georgia likely provides a favorable environment for various Vibrio species. From 2006 to 2009, the abundance, ecology, and diversity of clinically important Vibrio species w...

Effect of bacteriophages and chamber bitter (Phyllanthus amarus) in combination on Vibrio parahaemolyticus Truong Thi Bich Van, Do Tan Khang, Le Viet Dung, Hoang Bao Ngoc, Nguyen Pham Anh Thi, Tran Thanh Men, Vo Ngoc Tram Anh, Kim Thoa

Vibrios are natural inhabitants of aquatic environments and form symbiotic or pathogenic relationships with eukaryotic hosts. Recent studies reveal that the ability of vibrios to form biofilms (i.e. matrix-enclosed, surface-associated communities) depends upon specific structural genes (flagella, pili and exopolysaccharide biosynthesis) and regulatory processes (two-component regulators, quorum...

Mutants of Vibrio parahaemolyticus defective in binding activity for adenosine 3',5'-cyclic monophosphate are described. They were selected with medium containing the nucleotide together with starch.