Vibrio cholerae, a member of the family Vibrionaceae, is a facultatively anaerobic, Gram-negative, non-spore-forming curved rod, about 1.4–2.6 mm long, capable of respiratory and fermentative metabolism; it is well defined on the basis of biochemical tests and DNA homology studies (Baumann, Furniss & Lee, 1984). The bacterium is oxidase-positive, reduces nitrate, and is motile by means of a sin...

objective(s) amongst the various antibiotic resistant elements in vibrio. cholerae, sxt constin (sxt-c) is important. we were going to design a quick method for determination of antibiotic resistance gene pattern in sxt-c. materials and methods ninety four v. cholerae o1el tor isolates were used in this study. antibiotic susceptibility testing, multiplex pcr amplification of sxt-c containing df...

Genomic data generated from clinical Vibrio cholerae O1 isolates collected over a five year period in an area of Kolkata, India with seasonal cholera outbreaks allowed a detailed genetic analysis of serotype switching that occurred from Ogawa to Inaba and back to Ogawa. The change from Ogawa to Inaba resulted from mutational disruption of the methyltransferase encoded by the wbeT gene. Re-emerg...

A non-pathogenic "indicator" organism to relapse Vibrio cholerae in the routine quality control of TCBS medium was sought among a large collection of freeze-dried vibrios isolated mostly from environmental sources. One strain, which was consistently more sensitive to inhibition of growth on TCBS medium that strains of V. cholerae and V. parahaemolyticus, is recommended for this purpose. It has ...

Introduction Pathogenic Vibrio species are of major concern as they are responsible for infectious diseases in humans. However, other Vibrio species found in the aquatic environment and defined as non pathogenic for humans, such as Vibrio alginolyticus, Vibrio harveyi, Vibrio anguillarum, Vibrio fluvialis and others are also of interest in that, although rarely, they have been isolated in assoc...

Vibrio cholerae O1 is one of two serogroups responsible for epidemic cholera, a severe watery diarrhea that occurs after the bacterium colonizes the human small intestine and secretes a potent ADP-ribosylating toxin. Immunity to cholera is associated with intestinal anti-lipopolysaccharide (LPS) antibodies, which are known to inhibit V. cholerae motility and promote bacterial cell-cell crosslin...

Quantification of Vibrio spp. and abiotic environmental factors on the proliferation and longevity of V. parahaemolyticus and V. cholerae was studied. Water samples were collected onboard CRV Sagar Purvi from Veli, Neendakara, Kochi, Calicut and Kasaragod along the Kerala coast for hydrological and microbiological parameters. Pearson correlation and regression analysis were done for evaluating ...

Toxigenic strains of Vibrio cholerae, although not endemic in New Zealand, are isolated occasionally from recent overseas travellers. Isolates should be confirmed biochemically and serologically and the presence of cholera toxin genes established by a reference laboratory, as non-toxigenic strains are found here in the summer months. Serotypes O1 and O139 are the causative agents of epidemic ch...

Vibrio cholerae O1 El Tor variant strains produced much more cholera toxin than did prototype El Tor strains. The amount of cholera toxin produced by El Tor variant strains both in vitro and in vivo was more or less equivalent to that produced by classical strains.

In August 2010, Pakistan experienced major floods and a subsequent cholera epidemic. To clarify the population dynamics and transmission of Vibrio cholerae in Pakistan, we sequenced the genomes of all V. cholerae O1 El Tor isolates and compared the sequences to a global collection of 146 V. cholerae strains. Within the global phylogeny, all isolates from Pakistan formed 2 new subclades (PSC-1 a...