In the event of a new influenza pandemic, vaccines whose antigenicities match those of circulating strains must be rapidly produced. Here, we established an alternative reverse genetics system for influenza virus using the canine polymerase I (PolI) promoter sequence that works efficiently in the Madin-Darby canine kidney cell line, a cell line approved for human vaccine production. Using this ...

The oncolytic herpes simplex virus (HSV) that has been approved for clinical practice and those HSVs in clinical trials are attenuated viruses, often with the neurovirulence gene γ134.5 and additional genes deleted. One strategy to engineer nonattenuated oncolytic HSVs consists of retargeting the viral tropism to a cancer-specific receptor of choice, exemplified by HER2 (human epidermal growth ...

The bicyclo compounds possess a wide variety of activities so, in the present study we have develop a novel and simple method for the synthesis of such 3, 4-diphenyl-2, 5-diaza-bicyclo [4.3.1] decane derivatives. All the compounds synthesized were characterized by running TLC, IR, NMR and LCMS spectra. All the title compounds were screened for the determination of Cytotoxicity by MTT and SRB as...

BACKGROUND The phytochemical and pharmacological activities of Annona reticulata components suggest a wide range of clinical application in lieu of cancer chemotherapy. MATERIALS AND METHODS Ethanol and aqueous extracts of roots of Annona reticulata Linn were studied for their in vitro antiproliferative activity on A-549 (human lung carcinoma), K-562 (human chronic myelogenous leukemia bone m...

Objective(s): Oncolytic Herpes simplex virus type 1 (HSV-1) has emerged as a promising strategy for cancer therapy. However, development of novel oncolytic mutants has remained a major challenge owing to low efficiency of conventional genome editing methods. Recently, CRISPR-Cas9 has revolutionized genome editing.Materials and Methods: I...

the anti-cancer activity of metal ions in the lanthanide group is being considered recently. it has been reported that cerium salts might stimulate the metabolism and therefore, produce anti-cancer effects. however, little is known about the effects of protein-cerium complex in controlling cancer cell growth. the aim of the present study was to elucidate the possible pathways for the cytotoxic ...

Background In cell-based processes, and particularly in viral vaccine production, cell growth and death are strategic informations to obtain for process monitoring (ie. scale-up, determination of MOI, TOI, and harvest time). Dielectric spectroscopy is a tool which was increasingly implemented on cell-culture bioreactors as it presents great potentials, compared to other methods, for the in-line...