conclusions our results suggested that cb-nk cells progenitors could proliferate and differentiate on feeder layer of amnion membrane mscs in combination with specific cytokines to produce nk cells for immunotherapy. results combination of cytokines and msc layer yielded significant fold expansion in comparison with cytokines without feeder conditions (day 7: 5.2 ± 1.12 and 2 ± 0.78, respective...

Gene-engineered immune cell therapies have partially transformed cancer treatment, as exemplified by the use of chimeric antigen receptor (CAR)-T cells in certain hematologic malignancies. However, there are several limitations that need to be addressed target more types. Natural killer (NK) a type innate represent unique biology surveillance. In particular, NK obtained from heathy donors can s...

NK/T-cell ratios predict disease activity in relapsing remitting multiple sclerosis (RRMS). We investigated 50 RRMS patients whether interleukin-2 receptor alpha-chain (IL-2R?) expression and shedding associates with balance, as suggested by daclizumab-trials RRMS. A subsample (N = 31) was genotyped for IL2RA-associated MS risk SNPs. CD56bright NK-cell/IL-17A+CD4+ T-cell correlated negatively p...

BACKGROUND Uterine lymphoid cell repertoires are specialized in order to meet the twin demands of successful pregnancy and local immunosurveillance. The possibility that some of these populations might differentiate locally from progenitor cells has been proposed. METHODS Endometrial tissue from women with a history of infertility as well as fertile controls was examined for haematopoietic st...

Angiogenesis is essential for endometrial growth and repair, and disruption of this process may lead to common disorders of women, including menorrhagia and endometriosis. In pregnancy, failure of the endometrial spiral arterioles to undergo remodeling leads to preeclampsia. Here we report that in addition to vascular endothelial growth factor A (VEGF-A), human endometrium expresses messenger r...

Shark cartilage is considered as a natural dietary supplement consisting of anti-angiogenic, immunostimulatory, and anti-inflammatory characteristics. Therefore, this study was designed to inspect the possibility that whitecheek shark (Carcharhinus dussumieri) cartilage proteins motivate expression of NKG2D, CXCR3, NKP46, and NKP44 receptors on natural killer cells and their activities against ...

STUDY QUESTION Is the endometrial mononuclear cell population in infertile patients altered in subjects with classical endometrial polyps (macropolyps) versus endometrial micropolyps that are hysteroscopically recognized as small uterine cavity protrusions? SUMMARY ANSWER Macropolypoid endometrium had a low density of pan-leukocytes, pan-T cells and natural killer (NK) cells, whereas micropol...

The major histocompatibility complex class I related chain (MIC) is a stress-inducible protein modulating the function of immune natural killer (NK) cells, a major leukocyte subset involved in proper trophoblast invasion and spiral artery remodeling. Aim of the study was to evaluate whether upregulation of soluble MIC (sMIC) may reflect immune disorders associated to vascular pregnancy diseases...

Tissue-specific NK cells are abundant in the pregnant uterus and interact with invading placental trophoblast cells that transform the maternal arteries to increase the fetoplacental blood supply. Genetic case-control studies have implicated killer cell Ig-like receptor (KIR) genes and their HLA ligands in pregnancy disorders characterized by failure of trophoblast arterial transformation. Acti...

Abstract The progression and metastatic capacity of solid tumors are strongly influenced by immune cells in the tumor microenvironment. In non–small cell lung cancer (NSCLC), accumulation anti-inflammatory tumor-associated macrophages (TAM) is associated with worse clinical outcome resistance to therapy. Here we investigated landscape NSCLC presence protumoral TAMs expressing macrophage recepto...