Purpose: To determine the inhibitory effect of curcumin on expression brain-derived neurotrophic factor (BDNF) in hippocampus mice with sleep deprivation.
 Methods: Expressions BDNF and miRNA-206 were evaluated using quantitative real time-polymerase chain reaction (qRT-PCR) a sleep-deprived mouse model. Interaction was assessed by dual luciferase qPCR) assays. The also determined qRT-PCR....

MicroRNAs (miRNAs) are small (approximately 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measu...

Environmental sampling has become a commonly accepted diagnostic technique for means of identifying breaks in biosecurity. However, environmental samples have yet to be validated reverse transcriptase real-time PCR (qRT-PCR) analysis and there is no standardization sample processing. Therefore, the objective this project was evaluate different types samples, whether processing prior qRT-PCR wou...

Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels. Calculating accurate mRNA levels requires important parameters such as reaction efficiency and the fractional cycle ...

BACKGROUND Both respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are important viral pathogens causing respiratory tract infection (RTI) in the pediatric population. However, the clinical manifestations of RSV and hMPV infections are similar. Therefore, a reliable and rapid diagnostic tool is needed for diagnostic performance. METHODS In order to optimize diagnosis efficienc...

Hantaan virus (HTNV) is a major zoonotic pathogen that causes hemorrhagic fever with renal syndrome (HFRS) in Asia, especially in China. Shaanxi province, which is located in northwest of China, is one of the areas in China most severely afflicted with HFRS epidemics annually. This study aims to establish a quantitative RT-PCR (qRT-PCR) assay to detect HTNV both in cell culture and clinical ser...