Binding of endothelial nitric oxide synthase (eNOS) to the chaperone protein, Hsp90, promotes coupled eNOS synthetic activity. Using resistance level pulmonary arteries (PRA) from 2-day-, 5- to 7-day-, and 12-day-old piglets, we tested the hypothesis that Hsp90-eNOS interactions are developmentally regulated in the early neonatal period. PRA were isolated for coimmunoprecipitation and immunoblo...

BACKGROUND Primary aldosteronism (PA), the most common secondary cause of hypertension, can be screened for using the aldosterone/renin ratio. This ratio is raised in PA and its accuracy depends on the ability to measure plasma renin at extremely low concentrations. METHODS We compared two different procedures for assessing plasma renin. The conventional method, which measures plasma renin ac...

Prorenin is an inactive form of the aspartic protease renin. Like pepsinogen, it is activated at low pH. The kinetics of acid activation of prorenin were studied in human amniotic fluid and plasma and in preparations of purified prorenin isolated from amniotic fluid and plasma. Conversion of prorenin (pR) into active renin (R) appeared to be a two-step process involving the generation of an int...

More than 70 species of mycobacteria have been defined, and some can cause disease in humans, especially in immunocompromised patients. Species identification in most clinical laboratories is based on phenotypic characteristics and biochemical tests and final results are obtained only after two to four weeks. Quick identification methods, by reducing time for diagnosis, could expedite instituti...

In many research studies involving the use of participatory tools, much of the information gathered is of a qualitative nature. Some of this will contribute to addressing specific research questions, while other parts provide a general understanding of peoples’ livelihoods and constraints. The aim of this paper is to focus on the former. The paper concentrates on some quantitative analysis appr...

Progressive retinal atrophy (PRA) in dogs is characterised by the degeneration of the photoreceptor cells of the retina, resulting in vision loss and eventually complete blindness. The condition affects more than 100 dog breeds and is known to be genetically heterogeneous between breeds. Around 19 mutations have now been identified that are associated with PRA in around 49 breeds, but for the m...

Older adults face unique challenges and have differing needs when navigating the urban environment. At same time, civil servants policymakers in cities are facing difficulties promoting age-friendly initiatives due to lack of knowledge, data, tools about for older adults. In this context, smart can play a key role increasing participation (i.e., 65 years older) by collecting data needed provide...