Roots of young soybean (Glycine max [L.] Merr.) plants (up to 25 days old) contain two distinct urease isozymes, which are separable by hydroxyapatite chromatography. These two urease species (URE1 and URE2) differ in: (a) electrophoretic mobility in native gels, (b) pH dependence, and (c) recognition by a monoclonal antibody specific for the seed ("embryo-specific") urease. By these parameters...

wachsenden Konzentrationen an B U d R bzw. BU steigen in beiden Fällen die Ausbeuten um den gleichen Betrag an. Be im B U wurden organische Peroxide nachgewiesen, die in saurem Medium eine Zeitlang stabil waren und sich zur Isodialursäure ( I D S ) umwandel ten 2 . D ie bei der BUdR-Radio lyse in neutralem M e d i u m intermediär nachzuweisenden Perox ide sind anderer Natur. Aus ihnen wird kein...

Covalent binding is an important mechanism for many drugs to gain its function. We developed a computational algorithm to model this chemical event and extended it to a web server, the CovalentDock Cloud, to make it accessible directly online without any local installation and configuration. It provides a simple yet user-friendly web interface to perform covalent docking experiments and analysi...

The efficacy of a mechanism-based enzyme inhibitor depends not only on the rate of covalent modification of the target enzyme, but also on the affinity with which the enzyme binds the inhibitor before covalent bond formation. Since proteinases have binding regions that extend many residues beyond the scissile peptide bond, specific and potent mechanism-based inhibitors of this class of enzyme c...

Non-covalent protein-carbohydrate interactions mediate molecular targeting in many biological processes. Prediction of non-covalent carbohydrate binding sites on protein surfaces not only provides insights into the functions of the query proteins; information on key carbohydrate-binding residues could suggest site-directed mutagenesis experiments, design therapeutics targeting carbohydrate-bind...

Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of urea into ammonia and carbon dioxide. Although the amino acid sequences of plant and bacterial ureases are closely related, some biological activities differ significantly. Plant ureases but not bacterial ureases possess insecticidal properties independent of its ureolyti...

Covalent binding of a reactive metabolite of clozapine to neutrophils or their precursors is thought to play a role in the development of clozapine-induced agranulocytosis. Immunoblotting studies with an anti-clozapine antiserum detected covalent binding of clozapine to human neutrophils in vitro when HOCl was used to generate clozapine reactive metabolite (major clozapine adducts of 31, 49, 58...

Weak interactions, such as those non-covalent interactions that occur in biological systems, are less well characterised than their strong, covalent counterparts. Here, we discuss associations between two or more molecules and consider the effect of interactions with solvent molecules (particularly water) and changes in the internal structure of the associating molecules on binding. We go on to...

Ubiquitous soybean urease, as opposed to the seed-specific urease, designates the seemingly identical ureolytic activities of suspension cultures and leaves. It also appears to be the basal urease in developing seeds of a variety, Itachi, which lacks the seed-specific urease (Polacco, Winkler 1984 Plant Physiol 74: 800-804). On native polyacrylamide gels the ureolytic activities in crude extrac...