Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called 'Single Virus Genomics', which enabl...

Soil organic carbon (SOC) is a potential indicator of soil quality and ecosystem sustainability. The present study aimed to evaluate SOC pools under major cropping systems Mayiladuthurai district Tamil Nadu. composite samples were collected from two depths (0-15 15-30 cm) by stratified random sampling analysed for pH, EC (Electrical conductivity), C fractions, inorganic permanganate oxidisable ...

Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emer...

The vast majority of environmental bacteria and archaea remain uncultivated, yet their genome sequences are rapidly becoming available through single cell sequencing technologies. Reconstructing metabolism is one common way to make use of genome sequences of ecologically important bacteria, but molecular evolutionary analysis is another approach that, while currently underused, can reveal impor...

Magnetotactic bacteria are characterized by the magnetosome, a membrane-bound, nano-sized organelle containing a magnetic crystal. This organelle is believed to help the bacteria to navigate along the geomagnetic field lines and find a preferred habitat for survival. The magnetosome is a biomineral structure highly controlled by the bacteria, composed of either magnetite (Fe3O4) or greigite (Fe...

Maintenance of soil health is foremost importance to sustain and increase crop productivity, while meeting the demand a rising global population. Soil microbiome gaining increasing attention as modulator health. Microbial communities confer traits living organism, which functions holistically conforms part plant holobiont, reassembling human-gut axis. Novel strategies in biostimulant developmen...

Thirty soil samples were collected from fields that have been used for cultivating watermelons and canning tomatoes over 10 years. The microplastics (MPs) within these separated with a density floatation method the use of sieves filters. found black originated agricultural mulch film (BMF) in cultivations. ATR-FTIR spectroscopy revealed are same material as virgin BMF polyethylene blank. SEM im...