Upon digestion with a streptococcal extracellular nuclease, yeast RNA yielded acid-insoluble core having increased carrier activity for streptolysin S. The carrier activity was found in minor fractions of the core which were eluted from a DEAE-cellulose column at higher salt concentrations. Upon gel filtration through a Sephadex G-75 column, the effective component (Fr. I) was eluted earlier th...

Statistical graphics play a crucial role in exploratory data analysis, model checking and diagnosis. Until recently there were no formal visual methods in place for determining statistical significance of findings. This changed, when Buja et al. [2009] conceptually introduced two protocols for formal tests of visual findings. In this paper we take this a step further by comparing the lineup pro...

The initial step in biosynthesis of the O-antigen of Salmonella typhimurium is the formation of a lipid-linked derivative of galactose l-phosphate, according to the reaction UDP-galactose + P-lipid ti galactose-1 -PP-lipid + UMP. The enzyme activity has been detected in the cell envelope fraction of Citrobocter strain 139 (04, 5, 12) as well as in wild type S. typhimurium strain LT-2, but is mo...

The pyrH-encoded uridine 5'-monophosphate kinase (UMPK) is involved in both de novo and salvage synthesis of DNA and RNA precursors. Here we describe Mycobacterium tuberculosis UMPK (MtUMPK) cloning and expression in Escherichia coli. N-terminal amino acid sequencing and electrospray ionization mass spectrometry analyses confirmed the identity of homogeneous MtUMPK. MtUMPK catalyzed the phospho...

A fluorescence spectroscopy method is described for studying association of RNA polymerase with DNA templates. Using double beam differential fluorescence at excitation and emission wavelengths of 285 and 335 nm, respectively, the new technique discriminates non-specific decrease of fluorescence intensity by addition of DNA from quenching of polymerase fluorescence by protein-nucleic acid inter...

One of the main issues in achieving optimal results is disproportion between workload and work capacity an individual. The constraint independently measuring individual's tasks for non-recurring implementation a process varies individuals. As pilot study, this research collected responses from 16 administrative staff 9 faculties academic centers at Universiti Malaysia Pahang (UMP) who were sele...

In this study, our aim was to assess public health risks caused by ambient air pollution in close proximity production facilities of “Ulba Metallurgical Plant” JSC (JSC “UMP”) located Ust-Kamenogorsk, Kazakhstan. The study relied on the risk assessment methodology under exposure chemical environmental factors. Our research objects were Metal-lurgical and its effects health. We assessed “UMP”. d...

The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain (tentatively called II#2) was obtained, the EPA and DHA contents of which (in...