The cytoplasm of the megakaryocyte in the rat spleen possesses three zones, the perinuclear, intermediate and marginal. The perinuclear zone is characterized by the presence of Golgi apparatus, ribosomes, endoplasmic reticulum, and mitochondria. These organelles are found also in the more voluminous intermediate zone which in addition exhibits platelet granules and an extensive development of v...

Because of the limitations inherent in conventional transmission electron microscopy (TEM) using epoxy ultrathin sections for a clear recognition of biological entities having electron densities similar to or lower than that of epoxy resin, embedment-free sectioning for TEM has been developed. Embedment-free section TEM is reliably performed using water-soluble polyethylene glycol (PEG) as a tr...

Anderson, Douglas R. (National Cancer Institute, Bethesda, Md.), and Michael F. Barile. Ultrastructure of Mycoplasma hominis. J. Bacteriol. 90:180-192. 1965.-Both thin-sectioning and negative staining were used in an electron microscopic study of the morphology of pleuropneumonia-like organism (PPLO) strain HEp-2 (Mycoplasma hominis, type I) grown in an artificial liquid medium. The morphology ...

Cross-sectioned and cross-fractured crayfish axons display regions in which axon and Schwann cell surface membranes are regularly curved and project into the axoplasm. At these regions (projections) the two membranes run precisely parallel, separated by a gap of 130-140 A. Longitudinal fractures through the axons expose the inner fractured surface of either the internal (face A) or the external...

Blastocladia pringsheimii Reinsch is a zoosporic fungus with a strongly fermentative mode of metabolism. Mitochondria were observed by the electron microscope in thin sections of cells grown aerobically on either glycerol or glucose as carbon sources. The ultrastructure of the cristae was similar to that previously observed in Blastocladia ramose Thaxter, which is also strongly fermentative but...

Rhizophlyctis rosea (Chytridiomycota) is an apparently ubiquitous, soil-inhabiting, cellulose-degrading chytrid that is the type for Rhizophlyctis. Previous studies have revealed multiple zoospore subtypes among morphologically indistinguishable isolates in the R. rosea complex sensu Barr. In this study we analysed zoospore ultrastructure and combined nu-rRNA gene sequences (partial LSU and com...

Primary ciliary dyskinesia (PCD) is a rare genetic autosomal recessive disease associated with defect in the ultrastructure of epithelial cilia. Currently, there no standard method for diagnosing PCD, so diagnosis based on clinical picture and results tests, such as DNA diagnostics, nasal nitric oxide measurements, beat frequency biopsy, ultrastructure, etc. Diagnosis PCD can be difficult due t...

The morphology, immunohistochemistry, ultrastructure and natural history of sarcoma-like mural nodules in two serous cystic ovarian tumours are described.

The reliability of structures observed with electron microscope techniques represents a general problem in ultrastructure research . Obviously, every technique employed will have its limitations . Unfortunately the limitations are incompletely understood, and the eventual image obtained by electron microscopy deviates to an unknown extent from reality . The experimental approach to this problem...

Rode, L. J. (The University of Texas, Austin), and M. Glenn Williams. Utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments. J. Bacteriol. 92:1772-1778. 1966.-Spores of Bacillus megaterium are partially dissolved by sodium hypochlorite. Spore integuments become visible during the dissolution, and ultrastructural features may be detected. Three distinct integumen...