The inverted repeats of Tn5 are functionally different. One repeat codes for larger polypeptides, which are required for transposition. The other repeat has a better promoter for the neomycin resistance gene in the region of the repeat near the unique sequences. These dissimilarities are now shown to be caused by a single base pair difference. This change both creates a better promoter sequence...

Deviant genetic codes reported in ciliates share the same feature: one (UGA) or two (UAR) of the three canonical stop codons are translated into one particular amino acid. In many genera, such as Oxytricha, Paramecium, and Tetrahymena, UAR codons are translated into glutamine. UGA is translated into cysteine in Euplotes or into tryptophan in Colpoda inflata and Blepharisma americanum. Here, we ...

Aim. To follow the distribution and evolution of macaques genus-specific Alu repeat AluMacYa3 in Cercopithecidae MGMT gene orthologs. Methods. The homology between nucleotide sequences has been determined by BLAST 2.6.1 program. results search identification MGE have obtained CENSOR Results. It shown on example orthologs that is present not only intron macaques, but also other representatives O...

Anatomical characteristics regenerant plantlet of Arabica coffee (Coffea arabica L.) were observed to determine the difference performance between Sigararutang and Maragogige grown in shooting rooting medium. Transverse sections fresh roots, stems leaves three-month-old plantlets from somatic embryos collected used for study. Maragogipe as materials chosen based on bean size origin. Stomata mic...

RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...

The determination of nucleotide sequences in DNA, sequencing chloroplast genomes techniques, based on the use combinations a significant number genetic markers, as well nuclear genome are widely for inventory rare plant species, ecological monitoring and species diversity assessment. results our molecular analysis used to examine taxonomic identity phylogenetic relationship studied Iris collect...