Incubation of cultured human umbilical vein endothelial cells with [1-14C]-arachidonic acid, followed by RP-HPLC analysis, resulted in the appearance of two principal radioactive products besides 6-keto-PGF1 alpha. The first peak was HHT, a hydrolysis product of the prostaglandin endoperoxide. The second peak was esterified, converted to the trimethylsilyl ether derivative, and analyzed by GC/M...

a mild, efficient and fast method for the oxidation of alcohols and trimethylsilyl and tetrahydropyranyl ethers to their corresponding carbonyl compounds using cro3 in the presence of sulfonic acid functionalized ordered nanoporous na+-montmorillonite (sanm) and under solvent-free conditions is reported. all reactions were performed at room temperature in high to excellent yields. ease of the p...

1,1’-(Ethane-1,2-diyl)dipyridinium bistribromide (EDPBT) was used as a chemoselective and effective organocatalyst for the silylation of hydroxyl groups as well as desilylation of trimethylsilyl ethers under mild conditions at room temperature with good to excellent yields.

Pharmacognosy Research,2023,15,2,384-391.DOI:10.5530/pres.15.2.041Published:February 2023Type:Original Article Authors:Pooja Boora, Richa Puri, Sushila Rani, Monika Mehta, and Dechan Angmo Author(s) affiliations:Pooja Boora*, Department of Botany, Panjab University, Chandigarh, INDIA. Abstract:Background: Asthma is a very common chronic disease in which there an inflammation the lungs. Inflamma...

Rainbow trout macrophages maintained in short term culture when incubated with either calcium ionophore, A23187, or opsonized zymosan synthesize a range of lipoxygenase products including lipoxins and leukotrienes. These cells are unusual in that they generate more lipoxin than leukotriene following such challenge. The main lipoxin synthesized was lipoxin (LX) A4. This compound was identified b...

This paper describes a specific mass-fragmentographic method, involving a stable-isotope-labeled internal standard, for measurement of 24,25-dihydroxyvitamin D in human plasma. Vitamin D metabolites were rapidly extracted from plasma by using Sep-Pak C18 cartridges and separated into fractions on Sep-Pak SIL cartridges. The polar fraction, containing the dihydroxylated metabolites, was further ...

Phthalate esters, alkylphenols, bisphenol A and their chlorinated derivatives are the suspected endocrine disrupters or mutagens. These compounds, commonly called endocrine disrupter chemicals (EDCs), are widely used as plastic additives, lacquers, resins, or surfactants and can be usually found in environmental samples, namely wastewaters. An accurate and reproducible gas chromatographic-mass ...

A method was set up in which the primary prostaglandins (PGF2 alpha, PGD2, PGE2, thromboxane B2, 6-keto-PGF1 alpha and 6-keto-PGE1) and their catabolites (15-keto and 15-keto-13,14-dihydro) could be analyzed in the same sample at the same time. The method makes use of long capillary columns (60 m) to resolve the complex mixture during gas chromatography and mass fragmentography to provide the s...

The identification of epoxide regioisomers of arachidonic acid (EETs) as methyl esters is difficult because they coelute during gas chromatography and possess similar mass spectra. In the present study, EETs and their hydrolysis products, dihydroxyeicosatrienoic acids (DHETs), were analyzed as pentafluorobenzyl ester derivatives and their properties were compared to other esters. The four EET r...

This study was undertaken to elucidate the dominant endogenous gibberellins (GA) and the changes in their levels in germinating barley during malting. Also, a method for determining gibberellins in barley and malt by combined gas chromatography-mass spectrometry (GC-MS) was developed. Endogenous gibberellins in germinating Fuji Nijo I I and Betzes barley were sequentially extracted with ethanol...