نتایج جستجو برای: transfection
تعداد نتایج: 21797 فیلتر نتایج به سال:
MicroRNA (miRNA) regulation is a novel approach to manipulating the fate of mesenchymal stem cells, but an easy, safe, and highly efficient method of transfection is required. In this study, we developed an miRNA reverse transfection formulation by lyophilizing Lipofectamine 2000-miRNA lipoplexes on a tissue culture plate. The lipoplexes can be immobilized on a tissue culture plate with an inta...
The development of a T7 RNA polymerase (T7 RNAP) expressing cell line i.e. BSR T7/5 cells marks an improvement reverse genetics for the recovery recombinant Newcastle disease virus (rNDV). is developed by transient transfection plasmid encoding RNAP gene rNDV rescue. However, expression decreases gradually over multiple passages and eventually hinders rescue rNDV. To address this issue, lentivi...
Abstract Bispecific antibodies (bsAbs) are often composed of more than two component chains, such as Fabs-in-tandem immunoglobin (FIT-Ig) comprising three different which bring challenges for generating a high proportion the correctly assembled bsAbs in stable cell line. During CHO-K1 line construction FIT-Ig, we investigated FIT-Ig chain ratio transfection, where sets expression vectors were d...
A major obstacle in applying gene therapy to clinical practice is the lack of efficient and safe gene delivery techniques. Viral delivery has encountered a number of serious problems including immunological reactions and malignancy. Non-viral delivery methods (liposomes, sonoporation and electroporation) have either low efficiency invivo or produce severe collateral damage to ocular tissues. We...
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