Immune responses are orchestrated by controlling the initiation, magnitude, and duration of various signaling pathways. Adaptor proteins act as positive or negative regulators targeting critical molecules cascades. Signal-transducing adaptor protein-2 (STAP-2) contains typical features proteins, like a pleckstrin homology (PH) domain in N-terminal region Src 2 (SH2) central region. STAP-2 binds...

a:4:{s:10:"Background";s:1707:"Early embryonic development in mammals begins in transcriptional silence with an oocyte-mediated transcriptional reprogramming of parental gametes occurs during a so called across-the-board process of “erase-and-rebuild”. In this process, the parental transcription programs are erased long before (maternal) or soon thereafter (paternal) fertilization to generate a...

Objective(s): MicroRNAs (miRNAs) are considered as powerful, post-transcriptional regulators of gene expression in hepatocellular carcinoma cells (HCC). However, the function of miR-92a is still unclear in HCC. Materials and Methods: Expression of miR-92a in human HCC cell lines was evaluated using qRT-PCR. MTT assay and transwell assay were used to examine the function of miR-92a in HepG2 and ...

objective(s):oct4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. therefore, the effective expression regulation of this gene is highly critical. utr regions are of great significance to gene regulation. in this study, we aimed to investigate the potential regulatory role played by 5´ut...

Abstract BACKGROUND Aberrant activity of the H3K27 modifiers EZH2 and BRD4 is an important oncogenic driver for atypical teratoid/rhabdoid tumor (AT/RT), each potentially a possible therapeutic target treating AT/RT. Thus, targeting distinct histone modifier activities would be effective approach METHODS The effects inhibition on modification, cell proliferation, invasion were analyzed by immun...

Abstract The cell of origin Group 3 medulloblastoma, the most malignant medulloblastoma subgroup, is currently unknown and remains controversial. Transcriptional profiling has revealed that medulloblastomas are characterized by elevated expression a photoreceptor program, which not been described in normal cerebellar development but well developing retina pineal gland. We used lineage tracing s...

Barrier elements that are able to block the propagation of transcriptional silencing in yeast are functionally similar to chromatin boundary/insulator elements in metazoans that delimit functional chromosomal domains. We show that the upstream activating sequences of many highly expressed ribosome protein genes and glycolytic genes exhibit barrier activity. Analyses of these barriers indicate t...

mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

in the present study, an attempt was made to develop galactosylated albumin nanoparticles of cimetidine for treatment of acetaminophen induced hepatotoxicity. by developing the galactosylated nanoparticulated delivery of cimetidine the required action of drug at the target site i.e at liver can be provided. the advantage of targeting helps to reduce the systemic side effects which may be occur ...

The repression of transposable elements in eukaryotes often involves their transcriptional silencing via targeted chromatin modifications. In animal gonads, nuclear Argonaute proteins of the PIWI clade complexed with small guide RNAs (piRNAs) serve as sequence specificity determinants in this process. How binding of nuclear PIWI-piRNA complexes to nascent transcripts orchestrates heterochromati...