Isolation of pure and intact RNA is essential, but the concentration and integrity of total RNA preparations is affected by the purification process used. Applications for which RNA will ultimately be used may include reverse transcriptase (RT)-PCR, preparations of targets for microarrays, ribonuclease protection assays, preparations of cDNA libraries and northern blotting. Recently, the import...

To isolate high quality total RNA from plants of purple-fleshed sweet potato (Ipomoea batatas L. Lam) is difficult because these plants contain high levels of carbohydrates and pigment compounds. In this study, we developed a quick and reliable method for total RNA extraction. Using this extraction protocol, which was derived from the traditional CTAB method with modification, we obtained high ...

Ecological assessments are necessary to evaluate the status of our deteriorating ecosystems, however, assessment methods traditionally omit most microbes because unicellular organisms challenging identify. This omission is not ideal, as might be better indicators for changes in environmental conditions than taxa used. DNA- and RNA-based techniques increasingly applied ecological overcome this c...

Background and Aims: Different hepatitis C virus (HCV) genotypes have characteristic geographical distribution. Identification of HCV genotype is an important factor in the progression, clinical outcome and therapy of HCV infection. The aim of this study was to determine the prevalence of HCV genotypes among HCV-RNA positive patients in Yazd, Iran. Materials and Methods: In this cross-sectiona...

This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information.Multiple recent publications on RNA sequencing (RNA-seq) have demonstrated the power of next-generation sequencing technologies in whole-transcriptome analysis. Vendor-specific protocols used for RNA library construction often require at least 100 ng total RNA. However, under certain condition...

Recent RNA-seq studies reveal that the transcriptomes in animals and plants are more complex than previously thought, leading to the inclusion of many more splice isoforms in annotated genomes. However, it is possible that a significant proportion of the transcripts are spurious isoforms that do not contribute to functional proteins. One of the current hypotheses is that commonly used mRNA extr...

  Background & Objectives: Antisense peptide nucleic acids (PNA) that target growth essential genes show potent bactericidal properties without cell lysis. We considered the possibility that whether PNA treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to Dendritic cells (DCs). DCs are the most potent antigen presenting cells in th...

background the rate of mother-to-infant hepatitis c virus (hcv) transmission is relatively low, however, this mode of infection is the most important route for childhood hcv involvement. the chances of transmission are increased by the level of maternal hcv viraemia and maternal human immunodeficiency virus (hiv) co infection. objectives the objective of the present study was to determine the p...