Bacterial and mammalian ferritins are known to bind heme. The use of α-casein and biotinylated hemin could be applicable to detection of protein-bound heme and of proteins with heme-binding capacity, respectively. Although commercial horse spleen ferritin and purified horse spleen ferritin (L:H subunit ratio=4) bound to an α-casein-coated plate, and this binding could be inhibited by hemin, rec...

Background: Iron deficiency anemia (IDA) is the most common micronutrient deficiency in the world and almost a third of the world's population is involved with IDA. It even in mild type, could lead to serious disorders such as impairment of learning and mental focus. Reduction of iron resources results in an imbalance in the physiological function. With regard to the role of iron in myelination...

The serum transferrin concentration is usually overestimated when assessed as total iron binding capacity, because iron binds not only to transferrin but also, non-specifically, to other serum proteins [l-3]. Transferrin concentration can be measured directly by immunochemical methods including: radial immunodiffusion [ 1,2,4-61, immunonephelometric assay [6,7] and immunoturbidimetric assay [8,...

1 Department of Pathology and Laboratory Medicine, Santa Clara Valley Medical Center, San Jose, CA. * Address correspondence to this author at: Santa Clara Valley Medical Center, Department of Pathology and Laboratory Medicine, 751 S. Bascom Ave, San Jose, CA 95128. E-mail [email protected]. Received October 1, 2014; accepted December 11, 2014. DOI: 10.1373/clinchem.2014.234229 © ...

Ferritin was isolated from six Morris hepatomas with growth rates ranging from 3 to 12 days doubling time and also from regenerating rat liver. The ferritins from all six tumors differed electrophoretically from normal rat liver ferritin. Five of them migrated identically, and antigenic studies on four confirmed the change in protein structure. In response to iron treatment of the host, more fe...

Helicobacter pylori is related to iron deficiency anemia (IDA) and inflammatory responses causing gastric and duodenal ulcer and carcinoma. Moreover, it leads to deficiency of factors associated with iron adsorption and transfer. In the present study, we recruited 100 children (50 infected with H. pylori and 50 controls) aged 3-14 years old (40% male and 60% female) to evaluate the effect of H....

The aim of this observational study was to biochemically characterize the anaemia in GCA (giant cell arteritis) and PMR (polymyalgia rheumatica) patients. Values for mean corpuscular volume, mean corpuscular hemoglobin and soluble transferrin receptor were normal, whereas serum iron and total iron binding capacity (TIBC) were subnormal, and mean ferritin was above the upper reference limit. Iro...

BACKGROUND Firefighters are frequently exposed to significant concentrations of hazardous materials including heavy metals, aldehydes, hydrogen chloride, dichlorofluoromethane and some particulates. Many of these materials have been implicated in the triggering of several diseases. The aim of the present study is to investigate the effect of fire smoke exposure on serum heavy metals and possibl...

1. The effect of iron chelators on iron uptake, ferritin and total protein synthesis was studied in cultured Chang cells. Desferrioxamine depressed ferritin synthesis and completely inhibited iron uptake by ferritin protein. Rhodotorulic acid reduced iron uptake by the cells but had little effect on ferritin synthesis. Diethylenetriamine pentaacetic acid produced complete inhibition of iron upt...