Mono-ubiquitination of Fancd2 is essential for repairing DNA interstrand cross-links (ICLs), but the underlying mechanisms are unclear. The Fan1 nuclease, also required for ICL repair, is recruited to ICLs by ubiquitinated (Ub) Fancd2. This could in principle explain how Ub-Fancd2 promotes ICL repair, but we show that recruitment of Fan1 by Ub-Fancd2 is dispensable for ICL repair. Instead, Fan1...

We are undertaking a program to measure the characteristics of the intracluster light (total flux, profile, color, and substructure) in a sample of 10 galaxy clusters with a range of cluster mass, morphology, and redshift. We present here the methods and results for the first cluster in that sample, A3888. We have identified an intracluster light (ICL) component in A3888 in V and r that contain...

DNA interstrand cross-links (ICLs) are induced by many carcinogens and anitcancer drugs. ICL is a covalent linkage of both strands of DNA, preventing DNA strand separation during transcription and replication; thus, it is extremely cytotoxic in vivo. Psoralen and its derivatives are widely applied for the clinical treatment of several skin diseases and cutaneous T cell lymphoma, and they are al...

Mus81 plays an integral role in the maintenance of genome stability and DNA repair in mammalian cells. Deficiency of Mus81 in human and mouse cells results in hypersensitivity to interstrand cross-linking (ICL) agents and elevated levels of genomic instability. Furthermore, Mus81-mutant mice are susceptible to spontaneous lymphomas. The role of cellular checkpoints in mediating the phenotypes o...

The Fanconi anemia pathway is required for repair of DNA interstrand cross-links (ICL). Fanconi anemia pathway–deficient cells are hypersensitive to DNA ICL–inducing drugs such as cisplatin. Conversely, hyperactivation of the Fanconi anemia pathway is a mechanism that may underlie cellular resistance to DNA ICL agents. Modulating FANCD2 monoubiquitination, a key step in the Fanconi anemia pathw...

The volume-sensitive chloride current (ICl,swell) is found in the mammalian myocardium and is activated by osmotic swelling. The goal of this study was to examine the importance of the tyrosine kinases focal adhesion kinase (FAK) and Src kinase in cardiac ICl,swell regulation. Neonatal rat ventricular myocytes were cultured on collagen membranes and infected with adenovirus expressing beta-gala...

Whole cell patch clamp recordings were made from cultured myenteric neurons taken from murine proximal colon. The micropipette contained Cs to remove K currents. Depolarization elicited a slowly activating time-dependent outward current (Itdo), whereas, repolarization was followed by a slowly deactivating tail current (Itail). Itdo and Itail were present in about 70% of neurons. We identified t...

In [GrSi2] we gave a canonical construction of degenerating families of varieties with effective anticanonical bundle. The central fibre X of such a degeneration is a union of toric varieties, glued pairwise torically along toric prime divisors. In particular, the notion of toric strata makes sense on the central fiber. A somewhat complementary feature of our degeneration is their toroidal natu...

purpose : the objective of this study was to evaluate the outcome of acrysof toric intraocular lenses (iols) implantation in cataract surgery for the correction of regular corneal astigmatism. methods : twenty eyes of 16 patients with cataract and regular corneal astigmatism of 1.00-4.00 diopter (d) underwent surgery. depending on the amount of corneal astigmatism, an acrysof toric iol of type ...

A toric integrable geodesic flow on a manifold M is a completely integrable geodesic flow such that the integrals generate a homogeneous torus action on the punctured cotangent bundle T ∗M \ M . A toric integrable manifold is a manifold which has a toric integrable geodesic flow. Toric integrable manifolds can be characterized as those whose cosphere bundle (the sphere bundle in the cotangent b...