Thermostability can be increased by introducing prolines at suitable sites in target proteins. In this study, we compared five thermostable alpha-glucosidases and the moderate thermostable alpha-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4. Based on the amino acid sequence alignment, four sites (Leu152, Asn208, Lys285, and Thr430) of TtGluA were chosen for proline substitution...

Although it has been widely used as a feed supplement to reduce manure phosphorus pollution of swine and poultry, Aspergillus niger PhyA phytase is unable to withstand heat inactivation during feed pelleting. Crystal structure comparisons with its close homolog, the thermostable Aspergillus fumigatus phytase (Afp), suggest associations of thermostability with several key residues (E35, S42, R16...

kinetic modeling is an important issue, whose objective is the accurate determination of the rates of various reactions taking place in a reacting system. this issue is a pivotal element in the process design and development particularly for novel processes which are based on reactions taking place between various types of species. the fischer tropsch (ft) reactions have been used as the kineti...

Protein thermostability has received growing attention in recent years. Little is known about the determinants of thermal resistance in individual protein families. However, it is known that the mechanism is family-dependent and not identical for all proteins. We present a multivariate statistical analysis to find the determinants of thermostability in one protein family, the serine hydroxymeth...

subtilisins and proteinase K.]) Many protein cngincering studjes on subtilisins6) show that amino acid substitution can change the substrate specificity, pH depencience. thermostability, and eatalytie activity. Subtilisin BPN' mutant N218S (Asn218 is rcplaced by serine) has more thermostability than the wild-type cnzyme;7) Asn218 is threc rcsidues fi'om one of the catalytic residues. Ser221. Th...

The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 was constructed by homology modeling using Archaeoglobus fulgidus esterase as a reference, and the thermostability-structure relationship was analyzed. Our results verified the predicted 3D structure of EstE1 and identified the ion pair networks and hydrophobic interactions that are critical determinants for the thermo...

Rapid-equilibrium rate equations are derived for the five different mechanisms for the enzymatic catalysis of A + B + C --> products using a computer. These rate equations are used to determine the minimum number of velocities required to estimate the values of the kinetic parameters. The rate equation for the completely ordered mechanism involves four kinetic parameters, and the rate equation ...