We present a case of a patient who received nitrous oxide on two occasions within a period of 8 weeks and who subsequently developed a diffuse myelopathy, characterized by upper extremity paresis, lower extremity paraplegia and neurogenic bladder. Laboratory testing revealed hyperhomocysteinaemia and low levels of vitamin B(12). Because of this uncommon clinical presentation, we analysed the pa...

A thermolabile direct hemolysin from an El Tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. In addition to its hemolytic activity, the hemolysin is cytotoxic, cardiotoxic, and rapidly lethal. In these respects it resembles the thermostable direct hemolysin/cytotoxin/cardiotoxin/lethal toxin of Vibrio parahaemolyticus and...

Aluminum nitride (AlN) film coatings have been obtained by a new technique of hybrid helikon-arc ion-plasma deposition. Possibility to combine the magnetic-filtered arc plasma deposition technique with a treatment in RF plasma of helicon discharge allowed us to deposit AlN coatings on thermolabile substrates, significantly increasing the deposition rate. A study of spectral properties of AlN fi...

The thermal stability of Newcastle disease virus has been characterized in terms of the rate constants for inactivation of hemagglutinating activity (HA), neuraminidase activity (NA), and infectivity. Inactivation of HA results in the concomitant loss of NA. Infectivity, however, is much more thermolabile. Disintegration of the virus particle is not responsible for the identical rate constants ...

Intravenous injection into rabbits and cats of an aqueous extract of hornet venom sac, produced a significant reduction in the intraocular pressure. The mean reduction of the ocular pressure was between 7 and 15 mm Hg within one hour past injection and it lasted 6-12 h or in several instances even more than 24 h. The effect is produced by some thermolabile high-molecular-weight fraction(s), is ...

pyrimidine-specific nucleosidases which degraded radiola beled precursors used for measuring DNA and RNA synthe sis. Results of studies reported herein, and findings of others, demonstrate that CLC as well as peripheral blood leuko cytes of healthy subjects and patients with uncontrolled lymphocytic or myelocytic leukmeia release nucleosidases, and possibly other enzymes, when these cells are m...

A study of 31 temperature-sensitive mutants of mouse cytomegalovirus has indicated that two mutants (tsm1, tsm31) may be defective in immediate-early/early functions, two (tsm2, tsm3) may be defective in early functions and six (tsm9, tsm18, tsm22, tsm23, tsm28, tsm30) may be defective in early/late functions while the remainder are late function-defective mutants as determined by temperature-s...

A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which is thermolabile. The formation of the ligase-adenylate intermediate is also temperature-sensitive in vitro. Two temperature-resistant revertants of the mutant contain normal amounts of a thermostab...

High titers of a noninducible bacteriocin were produced by Clostridium acetobutylicum in a molasses fermentation medium used for the industrial production of solvents. Release of the bacteriocin towards the end of the exponential growth phase was accompanied by lysis of the culture and inhibition of the production of solvents. The producer cells were sensitive to the bacteriocin, which only aff...