A bioassay using Phytophthora infestam was developed to determine whether inhibitory proteins are induced in pathogen-inoculated plants. Using this bioassay, AP24, a 24-kilodalton protein causing lysis of sporangia and growth inhibition of P. infestam, was purified from tobacco plants inoculated with tobacco mosaic virus. Analysis of the N-terminal amino acid sequence identified AP24 as the tha...

Various manufacturers especially in the chemical, pharmaceutical and food industries employ sugars in their products. Sugars can be extracted from plant or microbial sources or they can be chemically synthesized. The majority of sugars are purchased as crystalline white powders and the most common examples are glucose, sucrose, lactose, fructose, maltose and xylitol. Protein-based sweeteners su...

Gray blight (GB) is one of the most destructive diseases tea plants, causing considerable damage and productivity losses; however, dynamic roles defense genes during pathogen infection remain largely unclear. To explore numerous molecular interactions associated with GB stress in we employed transcriptome, sRNAome degradome sequencing from 1 to 13 days post-inoculation (dpi) at 3-day intervals....

Plant often responds to fungal pathogens by expressing a group of proteins known as pathogenesis-related proteins (PRs). The expression of PR is mediated through pathogen-induced signal-transduction pathways that are fine-tuned by phytohormones such as methyl jasmonate (MeJA). Here, we report functional characterization of an Ocimum basilicum PR5 family member (ObTLP1) that was identified from ...

Proteins in the intercellular fluid (IF) of healthy Lupinus albus leaves were characterized. Silver staining of the proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed more than 30 polypeptides, with the major ones having a molecular mass lower than 36 kD. After amino-terminal amino acid sequence analysis, one of the major polypeptides, IF4, was shown to hav...

BACKGROUND The thaumatin-like protein (TLP) Ole e 13 in raw olive fruit is responsible for occupational allergy in olive oil mill workers. However, these workers do not experience allergic symptoms after ingestion of edible olive. OBJECTIVES To analyze the presence of IgE-reactive TLP in raw and edible olive fruit and to assess the allergenic potency of both sources. METHODS The content of ...

the histone-like protein hu is the most-abundant dna-binding protein in bacteria. the hu protein non-specifically binds and bends dna as a hetero- or homodimer, and can participate in dna supercoiling and dna condensation. it also takes part in dna functions such as replication, recombination, and repair. hu does not recognize any specific sequences but shows a certain degree of specificity to ...

Specific radiation damage can be used to solve macromolecular structures using the radiation-damage-induced phasing (RIP) method. The method has been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets that were collected on a third-generation synchrotron undulator beamline with a highly attenuated beam. ...

White rust, caused by Albugo candida, is a serious pathogen of Brassica juncea (Indian mustard) and poses a potential hazard to the presently developing canola-quality B. juncea industry worldwide. A comparative proteomic study was undertaken to explore the molecular mechanisms that underlie the defence responses of Brassica juncea to white rust disease caused by the biotrophic oomycete Albugo ...