20 We have designed a hybrid peptide by combining sequences of human β-defensin-1 and 21 θ-defensin, in an attempt to generate a molecule that combines the diversity in structure and 22 biological activity of two different peptides to yield a promising therapeutic candidate. HBD-1 23 was chosen as it is a natural defensin of humans that is constitutively expressed but its 24 antibacterial activ...

Studies on the substrate specificity of the catalytic subunit of bovine skeletal muscle adenosine 3’:5’-monophosphate-dependent protein kinase (EC 2.7.1.37,ATP:protein phosphotransferase) have been made using the synthetic peptide, Leu-Arg-Arg-Ala-Ser-Leu-Gly, corresponding to part of the local phosphorylation site sequence reported for porcine liver pyruvate kinase. The V,,,,, and apparent K,,...

CD4+ T cells transfected with the C-terminal 130 aa of human IL-16 are rendered resistant to HIV infection. Whether the constitutively expressed IL-16 acts intracellularly, extracellularly, or both is not clear. To address this question and to further study the processing of IL-16, new constructs containing either the C-terminal 130 aa or the C-terminal 100 aa (PDZ-like motif) were constructed ...

can predict cardiac failure after cardiac surgery. Circ J 2007; 71: 79–83 20 Kerbaul F, Giorgi R, Oddoze C, et al. High concentrations of N-BNP are related to non-infectious severe SIRS associated with cardiovascular dysfunction occurring after off-pump coronary artery surgery. Br J Anaesth 2004; 93: 639–44 21 Guerin V, Ayed SB, Varnous S, et al. Release of brain natriuretic-related peptides (B...

A novel peptide having free radical scavenging activity was separated, using an on-line high-performance liquid chromatography (HPLC) - ABTS screening method, from bovine skim milk fermented by Lactococcus lactis SL6 (KCTC 11865BP). It was further purified using reverse phase-HPLC (RP-HPLC) and sequenced by RP-HPLC-tandem mass spectrometry. The amino acid sequence of the identified peptide was ...

INTRODUCTION AND OBJECTIVES Neprilysin breaks down numerous vasoactive peptides. The soluble form of neprilysin, which was recently identified in heart failure, is associated with cardiovascular outcomes. Within a multibiomarker strategy, we directly compared soluble neprilysin and N-terminal pro-B-type natriuretic peptide as risk stratifiers in a real-life cohort of heart failure patients. M...

INTRODUCTION AND OBJECTIVES Measurement of natriuretic peptides may be recommended prior to echocardiography in patients with suspected heart failure. Cut-off point for heart failure diagnosis in primary care is not well established. We aimed to assess the optimal diagnostic cut-off value of N-terminal pro-B-type natriuretic peptide on a community population attended in primary care. METHODS ...

Myosin light-chain kinase (MLCK) of smooth muscle is multifunctional, being composed of N-terminal actin-binding domain, central kinase domain, and C-terminal myosin-binding domain. The kinase domain is the best characterized; this domain activates the interaction of smooth-muscle myosin with actin by phosphorylating the myosin light chain. We have recently shown that the Met-1-Pro-41 sequence ...