Enterobacter aerogenes clinical isolate LOR was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. PCR and cloning experiments from this strain identified a novel TEM-type beta-lactamase (TEM-121) differing by five amino acid substitutions from beta-lactamase TEM-2 (Glu104Lys, Arg164Ser, Ala237Thr, Glu240Lys, an...

Eighty-five extended-spectrum beta-lactamase-producing Enterobacteriaceae from a Slovak hospital have been studied. SHV-2a was predominant, but other variants have been detected, namely, SHV-5, SHV-12, TEM-12, TEM-15, and TEM-132, which differed from TEM-1 by amino acid substitutions R164H, E240K, and I173V and had kinetic properties similar to those of TEM-28.

The TEM The TEM has a similar optical configuration to an optical microscope. A flood beam of electrons illuminates a thin sample. The electron transmitted through the sample are projected onto a viewing screen or camera for observation (Figure 1). Samples must be thin (around 100 nm) and the beam energies must be high. Electrons may either pass through the sample without being scattered or may...

fatigue behavior of a vanadium microalloy steel has been studied in this research. here, microstructure constituents were considered which essentially are dependent on the heat treatment conditions. the results of high cycle fatigue tests revealed that heat treatment procedure and cooling rates have significant effects on fatigue properties. optimal heat treatment cycle of the microalloyed stee...

background: nano-biotechnology is considered as one of the mainly vigorous field of research in new material science. recently, biosynthetic methods employing both biological microorganisms such as bacteria and fungus or plants extract have developed speedily as a trouble-free and feasible choice to obtain nanomaterials alternative to more complex chemical synthetic procedures. the particular d...

use of zinc and iron oxide are common in the desulfurization process from natural gas. in thisresearch, after determinations of the size and specifications through tem and xrd for each of the metaloxides, two different size ratios were used, 0.66 for zinc oxide and 0.142 for iron oxide. further, absorption of sulfur compounds in gas (hydrogen sulfide (h2s), carbonyl sulfide (cos), methyl mercap...

Transanal endoscopic microsurgery may be considered medically necessary for treatment of clinical T1 rectal adenocarcinomas than cannot be removed using other means of local excision and that meet all of the following criteria:  Located in the middle or upper part of the rectum  Well or moderately differentiated (G1 or G2)  Without lymphadenopathy or microscopic angiolymphatic invasion  Les...

One of the main limitations of imaging at high spatial and temporal resolution during in-situ TEM experiments is the frame rate of the camera being used to image the dynamic process. While the recent development of direct detectors has provided the hardware to achieve frame rates approaching 0.1ms, the cameras are expensive and must replace existing detectors. In this paper, we examine the use ...

Two clinical isolates of Escherichia coli, CF1179 and CF1295, were isolated from a patient hospitalized in the hematology unit of the University Hospital of Clermont-Ferrand, Clermont-Ferrand, France. They were resistant to penicillin-clavulanate combinations and to ceftazidime. The double-disk synergy test was positive only for isolate CF1179. Molecular comparison of the isolates showed that t...

This review describes use of transmission electron microscopy (TEM) in wood and fiber analysis. Analytical techniques and sample preparation methods are used to localize substructures of the cell wall polymers and are discussed in this review. The ultrastructural features of the wood cell walls, the structures formed by microfibrils, and the distribution of cell wall polymers, as revealed by TE...